2. Basic Clinical Mycology (I) Flashcards
Specimens for fungi ID can be refrigerated at
4° C
Temp of blood and CSF specimens for fungi ID
30-37°C
Temp for dermatologic specimens for fungi ID
15-30°C
Container for dermatologic specimens
Dry container
Biosafety hoods used in the mycology laboratory
Class 2 or 3
Type of disinfectant in the mycology laboratory
Phenol-based
3 methods for specimen processing
Direct inoculation
Concentration
Minced (homogenized)
Specimen processing method; adding several drops of specimen to media
Direct inoculation
Specimen processing method; the specimen is streaked if the media is solid
Direct iniculation
Specimens for direct inoculation
Bronchial brush/wash Aspirates CSF Swabs Body fluids Hairs Scrapings
Specimen processing method; large volumes can be concentratd by centrifugation
Concentration
Specimen for centrifugation
Body fluids
CSF
Urine
Specimen processing method; some solids must be “destroyed” to expose a buried pathogen to the media
Minced (homogenized)
Specimen for mincing (homogenizing)
Nails
Tissues
Biopsies
Primary culture media for fungi
Test tubes
Culture media for subculture of fungi
Petri dishes
Most common media for fungi
Sabouraud dextrose agar (SDA)
SDA with less glucose for growth of Blastomyces dermatitidis
Emmon’s modification
Fungi that grows on Emmon’s modification of SDA
Blastomyces dermatitidis
Inhibitory substances that may be present in fungal culture media
Chloramphenicol
Gentamicin
Cycloheximide
Media for selective recovery of dimorphs and dermatophytes
Mycosel and mycobiotic
Composition of mycosel and mycobiotic
SDA + chloramphenicol + cycloheximide
Fungi recovered using Mycosel and mycobiotic
Dimorphs
Dermatophytes
Enriched media enhanced for recovery of C. neoformans and dimorphic transitions Sporothrix and Paracoccidioides
Brain heart infusion (BHI) agar
BHI agar modification for Zygomycetes
Broth + penicillin
Fungi recovered from BHI agar (+ broth and penicillin modification)
C. neoformans
Sporothrix (dimorphic transition)
Paracoccidioides (dimorphic transition)
Zygomycetes
Media for C. neoformans in contaminated specimen
BHI + gentamicin + chloramphenicol
Fungi recovered from BHI + gentamicin + chloramphenicol
C. neoformans in contaminated specimen
Enriched medium for Cryptococcus spp. and thermally dimorphic fungi
Sabouraud-brain heart infusion agar (SABHI)
Fungi recovered from SABHI
- Cryptococcus spp.
- Thermally dimorphic fungi
Selective and differential media for presumptive ID of Candida from primary plates
CHROMagar Candida
Light to medium green on CHROMagar
Candida albicans
Light blue to metallic-blue on CHROMagar
Candida tropicalis
Loght rose with a whitish border on CHROMagar
Candida krusei
Media that contains inorganic salts, chloramphenicol, and gentamicin that inhibits bacteria
Inhibitory mold agar (IMA)
Fungi recovered for IMA
Cryptococcus spp.
Media for dermatophytes from heavily contaminated specimens (pink-to-red color change)
Dermatophyte test medium
Media for subculture
Potato dextrose agar (PDA)
Potato flake agar (PFA)
Media for yeast morphology
Cornmeal agar
Media to differentiate yeast morphology
Cornmeal agar
Incubation temp for obligate filamentous fungi
25 or 37°C
Incubation temp for dimorphic fungi
25 and 37°C
Incubation temp for yeasts
25 or 37°C
Incubation period of media
3-4 weeks
Media recommended for promoting sporulation
Cornmeal agar
Specimen for fungi pathogen must be processed within
2 hours
