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Elsevier Mycology (RVSP) > 2. Basic Clinical Mycology (I) > Flashcards

2. Basic Clinical Mycology (I) Flashcards

1
Q

Specimens for fungi ID can be refrigerated at

A

4° C

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2
Q

Temp of blood and CSF specimens for fungi ID

A

30-37°C

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3
Q

Temp for dermatologic specimens for fungi ID

A

15-30°C

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4
Q

Container for dermatologic specimens

A

Dry container

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5
Q

Biosafety hoods used in the mycology laboratory

A

Class 2 or 3

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6
Q

Type of disinfectant in the mycology laboratory

A

Phenol-based

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7
Q

3 methods for specimen processing

A

Direct inoculation
Concentration
Minced (homogenized)

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8
Q

Specimen processing method; adding several drops of specimen to media

A

Direct inoculation

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9
Q

Specimen processing method; the specimen is streaked if the media is solid

A

Direct iniculation

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10
Q

Specimens for direct inoculation

A 
Bronchial brush/wash
Aspirates
CSF
Swabs
Body fluids
Hairs
Scrapings
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11
Q

Specimen processing method; large volumes can be concentratd by centrifugation

A

Concentration

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12
Q

Specimen for centrifugation

A

Body fluids
CSF
Urine

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13
Q

Specimen processing method; some solids must be “destroyed” to expose a buried pathogen to the media

A

Minced (homogenized)

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14
Q

Specimen for mincing (homogenizing)

A

Nails
Tissues
Biopsies

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15
Q

Primary culture media for fungi

A

Test tubes

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16
Q

Culture media for subculture of fungi

A

Petri dishes

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17
Q

Most common media for fungi

A

Sabouraud dextrose agar (SDA)

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18
Q

SDA with less glucose for growth of Blastomyces dermatitidis

A

Emmon’s modification

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19
Q

Fungi that grows on Emmon’s modification of SDA

A

Blastomyces dermatitidis

20
Q

Inhibitory substances that may be present in fungal culture media

A

Chloramphenicol
Gentamicin
Cycloheximide

21
Q

Media for selective recovery of dimorphs and dermatophytes

A

Mycosel and mycobiotic

22
Q

Composition of mycosel and mycobiotic

A

SDA + chloramphenicol + cycloheximide

23
Q

Fungi recovered using Mycosel and mycobiotic

A

Dimorphs

Dermatophytes

24
Q

Enriched media enhanced for recovery of C. neoformans and dimorphic transitions Sporothrix and Paracoccidioides

A

Brain heart infusion (BHI) agar

25
Q

BHI agar modification for Zygomycetes

A

Broth + penicillin

26
Q

Fungi recovered from BHI agar (+ broth and penicillin modification)

A

C. neoformans
Sporothrix (dimorphic transition)
Paracoccidioides (dimorphic transition)
Zygomycetes

27
Q

Media for C. neoformans in contaminated specimen

A

BHI + gentamicin + chloramphenicol

28
Q

Fungi recovered from BHI + gentamicin + chloramphenicol

A

C. neoformans in contaminated specimen

29
Q

Enriched medium for Cryptococcus spp. and thermally dimorphic fungi

A

Sabouraud-brain heart infusion agar (SABHI)

30
Q

Fungi recovered from SABHI

A
  • Cryptococcus spp.

- Thermally dimorphic fungi

31
Q

Selective and differential media for presumptive ID of Candida from primary plates

A

CHROMagar Candida

32
Q

Light to medium green on CHROMagar

A

Candida albicans

33
Q

Light blue to metallic-blue on CHROMagar

A

Candida tropicalis

34
Q

Loght rose with a whitish border on CHROMagar

A

Candida krusei

35
Q

Media that contains inorganic salts, chloramphenicol, and gentamicin that inhibits bacteria

A

Inhibitory mold agar (IMA)

36
Q

Fungi recovered for IMA

A

Cryptococcus spp.

37
Q

Media for dermatophytes from heavily contaminated specimens (pink-to-red color change)

A

Dermatophyte test medium

38
Q

Media for subculture

A

Potato dextrose agar (PDA)

Potato flake agar (PFA)

39
Q

Media for yeast morphology

A

Cornmeal agar

40
Q

Media to differentiate yeast morphology

A

Cornmeal agar

41
Q

Incubation temp for obligate filamentous fungi

A

25 or 37°C

42
Q

Incubation temp for dimorphic fungi

A

25 and 37°C

43
Q

Incubation temp for yeasts

A

25 or 37°C

44
Q

Incubation period of media

A

3-4 weeks

45
Q

Media recommended for promoting sporulation

A

Cornmeal agar

46
Q

Specimen for fungi pathogen must be processed within

A

2 hours

Elsevier Mycology (RVSP) (13 decks)

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