2. Basic Clinical Mycology (I)

Question 1

Specimens for fungi ID can be refrigerated at

Answer 1

4° C

Question 2

Temp of blood and CSF specimens for fungi ID

Answer 2

30-37°C

Question 3

Temp for dermatologic specimens for fungi ID

Answer 3

15-30°C

Question 4

Container for dermatologic specimens

Answer 4

Dry container

Question 5

Biosafety hoods used in the mycology laboratory

Answer 5

Class 2 or 3

Question 6

Type of disinfectant in the mycology laboratory

Answer 6

Phenol-based

Question 7

3 methods for specimen processing

Answer 7

Direct inoculation
Concentration
Minced (homogenized)

Question 8

Specimen processing method; adding several drops of specimen to media

Answer 8

Direct inoculation

Question 9

Specimen processing method; the specimen is streaked if the media is solid

Answer 9

Direct iniculation

Question 10

Specimens for direct inoculation

Answer 10

Bronchial brush/wash
Aspirates
CSF
Swabs
Body fluids
Hairs
Scrapings

Question 11

Specimen processing method; large volumes can be concentratd by centrifugation

Answer 11

Concentration

Question 12

Specimen for centrifugation

Answer 12

Body fluids
CSF
Urine

Question 13

Specimen processing method; some solids must be “destroyed” to expose a buried pathogen to the media

Answer 13

Minced (homogenized)

Question 14

Specimen for mincing (homogenizing)

Answer 14

Nails
Tissues
Biopsies

Question 15

Primary culture media for fungi

Answer 15

Test tubes

Question 16

Culture media for subculture of fungi

Answer 16

Petri dishes

Question 17

Most common media for fungi

Answer 17

Sabouraud dextrose agar (SDA)

Question 18

SDA with less glucose for growth of Blastomyces dermatitidis

Answer 18

Emmon’s modification

Question 19

Fungi that grows on Emmon’s modification of SDA

Answer 19

Blastomyces dermatitidis

Question 20

Inhibitory substances that may be present in fungal culture media

Answer 20

Chloramphenicol
Gentamicin
Cycloheximide

Question 21

Media for selective recovery of dimorphs and dermatophytes

Answer 21

Mycosel and mycobiotic

Question 22

Composition of mycosel and mycobiotic

Answer 22

SDA + chloramphenicol + cycloheximide

Question 23

Fungi recovered using Mycosel and mycobiotic

Answer 23

Dimorphs

Dermatophytes

Question 24

Enriched media enhanced for recovery of C. neoformans and dimorphic transitions Sporothrix and Paracoccidioides

Answer 24

Brain heart infusion (BHI) agar

Question 25

BHI agar modification for Zygomycetes

Answer 25

Broth + penicillin

Question 26

Fungi recovered from BHI agar (+ broth and penicillin modification)

Answer 26

C. neoformans Sporothrix (dimorphic transition) Paracoccidioides (dimorphic transition) Zygomycetes

Question 27

Media for C. neoformans in contaminated specimen

Answer 27

BHI + gentamicin + chloramphenicol

Question 28

Fungi recovered from BHI + gentamicin + chloramphenicol

Answer 28

C. neoformans in contaminated specimen

Question 29

Enriched medium for Cryptococcus spp. and thermally dimorphic fungi

Answer 29

Sabouraud-brain heart infusion agar (SABHI)

Question 30

Fungi recovered from SABHI

Answer 30

- Cryptococcus spp. | - Thermally dimorphic fungi

Question 31

Selective and differential media for presumptive ID of Candida from primary plates

Answer 31

CHROMagar Candida

Question 32

Light to medium green on CHROMagar

Answer 32

Candida albicans

Question 33

Light blue to metallic-blue on CHROMagar

Answer 33

Candida tropicalis

Question 34

Loght rose with a whitish border on CHROMagar

Answer 34

Candida krusei

Question 35

Media that contains inorganic salts, chloramphenicol, and gentamicin that inhibits bacteria

Answer 35

Inhibitory mold agar (IMA)

Question 36

Fungi recovered for IMA

Answer 36

Cryptococcus spp.

Question 37

Media for dermatophytes from heavily contaminated specimens (pink-to-red color change)

Answer 37

Dermatophyte test medium

Question 38

Media for subculture

Answer 38

Potato dextrose agar (PDA) | Potato flake agar (PFA)

Question 39

Media for yeast morphology

Answer 39

Cornmeal agar

Question 40

Media to differentiate yeast morphology

Answer 40

Cornmeal agar

Question 41

Incubation temp for obligate filamentous fungi

Answer 41

25 or 37°C

Question 42

Incubation temp for dimorphic fungi

Answer 42

25 and 37°C

Question 43

Incubation temp for yeasts

Answer 43

25 or 37°C

Question 44

Incubation period of media

Answer 44

3-4 weeks

Question 45

Media recommended for promoting sporulation

Answer 45

Cornmeal agar

Question 46

Specimen for fungi pathogen must be processed within

Answer 46

2 hours