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Elsevier Mycology (RVSP) > 2. Basic Clinical Mycology (II) > Flashcards

2. Basic Clinical Mycology (II) Flashcards

1
Q

Percent of KOH used in KOH preparation

A

15% KOH

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2
Q

Added to KOH preparation that binds to cell wall and fluoresces blue-white under ultraviolet light

A

Calcofluor white

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3
Q

Used to visualize the capsule of C. neoformans in CSF; negative staining

A

India ink stain

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4
Q

India ink stain has been replaced with

A

Cryptococcal antigen test

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5
Q

Fungi stained with Giemsa, Wright-Giemsa

A

Histoplasmosis capsulatum (intracellular)

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6
Q

Color of fungi in H&E

A

Pink to pinkish-blue

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7
Q

Fungi stained with Meyer’s mucicarmine

A

C. neoformans

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8
Q

Color of C. neoformans in Meyer’s mucicarmine

A

Rose red

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9
Q

Stain for C. neoformans (rose red)

A

Meyer’s mucicarmine

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10
Q

Color of fungi in Gomori methenamine silver (GMS)

A

Black

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11
Q

Color of fungi in Papanicolaou stain

A

Pink to blue

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12
Q

Color of fungi in Periodic acid-Schiff (PAS)

A

Red or purple

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13
Q

Red or purple color

A

PAS

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14
Q

Pink to blue

A

Papanicolaou stain

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15
Q

Black

A

GMS

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16
Q

Rose red

A

Meyer’s mucicarmine

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17
Q

Pink to pinkish-blue

A

H&E

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18
Q

Growth period of yeasts

A

2-3 days

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19
Q

Growth period of molds

A

Rapid:

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20
Q

Dictates the texture of fungi colonies

A

Aerial hyphae

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21
Q

Leathery, waxy texture

22
Q

Suede, plush texture

23
Q

Texture that looks like Staphylococcus

24
Q

Fluffy texture

25
Q

Powdery texture

26
Q

Radial grooves, “folded” topography

27
Q

Central depression and raised edge topography

A

Crateriform

28
Q

Rough knobs topography

29
Q

Brainlike topography

A

Cerebriform

30
Q

Methods for examination of molds

A
  • tease/cut preparation
  • scotch tape preparation
  • slide culture
31
Q

Examination of molds; organism removed directly from culture plate using dissecting needle then placed on a slide

A

Tease/cut preparation

32
Q

Examination of molds; a scotch tape is used to transfer aerial hyphae from a colony to a microscope slide

A

Scotch tape preparation

33
Q

Examination of molds; organism is subcultured on a piece of agar then covered with coverslip, the organism grows onto coverslip

A

Slide culture

34
Q

Best method for examination of molds;

A

Slide culture

35
Q

Used to stain and preserve fungal elements in culture isolate

A

Lactophenol cotton blue (LPCB)

36
Q

Stain used for examination of molds

A

Lactophenol cotton blue (LPCB)

37
Q

3 methods for dermatophyte ID

A

Hair perforation
Urease test
Trichophyton agars
Rice grain growth

38
Q

How to do hair perforation for dermatophyte ID?

A
  • 5-10 mm sterile hair is floated on sterile water and yeast extract
  • conidia or hyphae are inoculated onto water surface
  • remove hair shafts and observe in LPCB weekly for 1 month
39
Q

Positive for hair perforation for dermatophyte ID

A

Trichophyton mentagrophytes

40
Q

Negative for hair perforation in dermatophyte ID

A

Trichophyton rubrum

41
Q

Observation time for hair perforation for dermatophyte ID

A

Weekly for 1 month

42
Q

Observation time for urease test for dermatophyte ID

A

5 days at RT

43
Q

Positive for urease test for dermatophyte ID

A

T. mentagrophytes

44
Q

Negative/weak for urease test for dermatophyte ID

45
Q

Trichophyton agars used in labs

A
  • Trichophyton agar 1 (without thiamine)

- Trichophyton agar 2 (with thiamine)

46
Q

Observation time for Trichophyton agars for dermatophyte ID

A

10-14 days

47
Q

Media for rice grain growth for dermatophyte ID

A

Sterile, nonfortified rice grain media

48
Q

Observation time for rice grain growth for dermatophyte ID

49
Q

Fungi differentiated in rice grain growth for dermatophyte ID

A

Microsporum canis

Microsporum audouinii

50
Q

Used to dissolve nonfungal materials in skin, nail, hair, fluids, exudates, and biopsy specimens

A

KOH preparation

Elsevier Mycology (RVSP) (13 decks)

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