6. Subcutaneous Mycoses Flashcards
3 types of conidiation of dematiaceous fungi
Cladosporium type
Phialophora type
Rhinocladiella type
Conidiation of dematiaceous fungi; resembles a tree, in which conidiophore is the trunk and branched chains of conidia form the branches
Cladosporium type
Conidiation of dematiaceous fungi; short conidiophores + phialide, vase shaped, conidia extruded from phialide and then cluster
Phialophora type
Conidiation of dematiaceous fungi; stalked conidiophores that become knobbly as conidia produced sequentially until a Cladosporium type of conidiation is reached
Rhinocladiella type
Specimens for subcutaneous fungi ID is collected by
Aspiration
Media for subcutaenous fungi ID
SDA
PDA (subculture)
A chronic granulomatous disease of feet (lower extremeties)
Mycetoma (Madura foot/Maduromycosis)
2 types of mycetoma
Eumycotic
Actinomycotic
Mycetoma granule; mycelium with hyphae 1 μm in diameter
Actinomycotic granule
Mycetoma granule; wide hyphae (2-4 μm) terminating in chlamydoconidia
Eumycotic granules
Verrucoid (wartlike) lesions on feet, legs, hands, and buttocks
Chromoblastomycosis
Microscopic:
Single-celled or clusters of single-cells
Dark pigment
Chromoblastomycosis
Culture temp and time for Chromoblastomycosis
6 weeks at RT
Subcutaneous mycosis that can have a systemic spread
Phaeohyphomycosis
Subcutaneous mycosis that uses the types of conidiation as a guide for ID
Chromoblastomycosis
Subcutaneous fungi that causes mycetoma
Pseudoallescheria boydii
Exophiala jeanselmei
Acremonium spp.
PEA
Subcutaneous fungi that causes Chromoblastomycosis
- Cladophialophora carrionii (Cladosporium type)
- Fonsecaea pedrosoi (Cladosporium, Phialophora, Rhinocladiella type)
- Fonsecaea compacta (Cladosporium type)
- Phialophora verrucosa (Phialophora type)
Subcutaneous fungi that causes Phaeohyphomycosis
Phialophora verrucosa
Fonsecaea pedrosoi
Exophiala jeanselmei
Wangiella dermatitidis
How many days will Cladophialophora carrionii grow?
30 days
Acquired when not wearing shoes, trauma
Cladophialophora carrionii
Colonies are grey-green to black on surface and cottony on reverse side within 30 days
Cladophialophora carrionii
Gray-green to black, cottony colony within 21 days
Fonsecaea pedrosoi
How many days will Fonsecaea pedrosoi grow?
21 days
Olive-green to black, velvety colonies
Phialophora verrucosa
Name for alternate asexual stage of Pseudoallescheria boydii
Scedosporium apiospermum
Macroscopic:
- rapid grower
- white to brownish-gray, fluffy colonies
Pseudoallescheria boydii
Microscopic:
- hyaline, septate hyphae
- single anelloconidia produced on an anellophore (conidiophore)
Pseudoallescheria boydii
Young cultures: black yeasts
Mature cultures: velvety colonies
Exophiala jeanselmei
Sticklike coniodiophores with clustered conidia
Exophiala jeanselmei
Optimum growth temp of Wangiella dermatitidis
40-42°C
Macroscopic:
- initially black yeast
- after 10 days, olive-gray to black velvety or glabrous colony
Wangiella dermatitidis
Microscopic:
- pigmented, septate hyphae
- conidiophores are indistinguishable from vegetative hyphae, except that conidia are clustered at ends
Wangiella dermatitidis
Has similar morphology to E. jeanselmei
Wangiella dermatitidis
Etiologic agent of mycetomas, corneal infections, and nail infections
Acremonium spp.
4 major infections under subcutaneous mycoses
Mycetoma
Chromoblastomycosis
Phaeohyphomycosis
Sporotrichosis
