8.2 replication of dna Flashcards
Leading Strand
The strand of DNA that heads towards the replication fork.
Lagging Strand
The strand of DNA that strays away from the replication fork.
Semi-Discontinous
short fragments of DNA are joined together subsequently by the DNA ligase to produce an uninterrupted DNA strand.
Semiconservative replication
Two strands of DNA spilt, allowing them to create new strands that will keep splitting.
Helicase
Rips apart DNA strands
Primase
Adds an RNA primer
DNA Polymerase III
DNA nucleotides are addded
DNA Polymerase I
RNA primer is removed, and replaced with DNA.
Lipase
Okaskai Fragments fused together.
Order of events in DNA replication:
- Helicase
- Primase
- DNA Polymerase III
- DNA Polymerase I
- Lipase
Explain Meselson and Stahl’s experimental setup and what was concluded from the results.
They found that DNA follows a semiconservative model- DNA will have a parental strand and a new strand ALWAYS.