8.1 Serologic Test for Streptococcal Disease

Question 1

Main etiologic Agent for Strep disease?

Answer 1

S. pyogenes

Question 2

Diseases: Strep throat; Scarlet fever; Impetigo; Erysipelas

What are the sequelae?

Answer 2

Glomerulonephritis (kidneys); Rheumatic Heart Disease (heart)

Question 3

Important pathogenic determinant of Strep for Serologic testing?

Answer 3

Streptolysin O
Exoenzymes

Question 4

Streptolysin O: Non immunogenic

Streptolysin S: Immunogenic

T or F

Answer 4

F

Baligtad

Question 5

Streptolysin O is a responsible for Sub surface hemolysis

T or F

Answer 5

T

Question 6

Streptolysin O: Oxygen labile

Streptolysin S: Oxygen Stable

T or F

Answer 6

T

Question 7

ASO Tube method Principle?

a. Passive Agglutination
b. Neutralization

Answer 7

b

Question 8

ASO tube method reagents includes:
- SLO Reagent
- PBS (Diluent)
- 5% _____
- NSS
- Distilled water

Answer 8

RCS

Question 9

Specimen for ASO Tube method?

Answer 9

Serum

Question 10

How many tube to set up ASO tube method?

Answer 10

12

Question 11

Tube 11: Red Cell control

Tube 12: SLO Control

T or F

Answer 11

T

Question 12

Tube 11: Positive Result
- No hemolysis

Tube 12: Negative result:
- Hemolysis

T or F

Answer 12

T

Question 13

1-10 tubes: Test
11-12: Control

T or F

Answer 13

T

Question 14

Tube dilution:

Tube 1: ______
Tube 2-6:______
Tube 7-10:______

Answer 14

1:10

1:100

1:500

Question 15

Checking of hemolysis is by adding red cells and SLO Reagents then incubate

T or F

Answer 15

T

Question 16

ASO Titer:

Reciprocal of highest dilution showing WITH Hemolysis

T or F

Answer 16

F

No

Question 17

Normal Values for Tube method:

Children: <______

Adults:<______

Answer 17

125 Todd units

166 Todd units

Question 18

Multienzyme test to detect antibodies against the different exoenzyme

Answer 18

Streptozyme test

Question 19

Streptozyme test is a Semi-quantitative, confirmatory test

T or F

Answer 19

F

Screening

Question 20

Specimen for Streptozyme

Answer 20

Serum

Question 21

Detection limit for Streptozyme test?

Answer 21

166 Todd units

Question 22

Components of Streptozyme Reagent?

Answer 22

Sheep RBC coated with enzyme

Question 23

Is it possible that the patient would be positive with streptozyme but negative for ASO?

Answer 23

Yes, because the streptozyme detects not only ASO but also other antibodies

Question 24

Streptozyme detects many antibodies related to Strep

T or F

Answer 24

T

Question 25

if Streptozyme is positive, ASO may be negative

T or F

Answer 25

T

It can be negative since not only SO can be detected but other enzymes

however if ASO is positive, expect Streptozyme to be positive

Question 26

ASO Slide method Principle?

a. Passive Agglutination
b. Neutralization

Answer 26

a

Question 27

Specimen for ASO Slide test?

Answer 27

Serum 40uL

Question 28

Detection limit for ASO Slide test?

Answer 28

200 IU/mL

Question 29

ASO slide test is a Sensitivity test

T or F

Answer 29

T

Question 30

Reagent for ASO sLide test?

Answer 30

Latex particle coated with SLO

Question 31

Analyte of Interest for ASO Slide test?

Answer 31

Ab

Question 32

Positive result for ASO Slide test?

Answer 32

Agglutination