6.4 Venereal Disease Research Laboratory Test

Question 1

Standard and Classical tests for detction of Reagin

Answer 1

VDRL

Question 2

What is the immunologic principle of VDRL

Answer 2

Microscopic Flocculation

Question 3

What is the analyte being detected in VDRL?

Answer 3

Reagin

Question 4

What are the components of Antigen Suspension?

Ca_________
L___________
Ch___________
1%_____________
B_________

Answer 4

Cardiolipin
Lecithin
Cholesterol
1% Benzoic Acid
Buffer

Question 5

Dissolving medium

a. Cardiolipin
b. Lecithin
c. Cholesterol
d. 1% Benzoic Acid
e. Buffer

Answer 5

b

Question 6

Center pg absorption

a. Cardiolipin
b. Lecithin
c. Cholesterol
d. 1% Benzoic Acid
e. Buffer

Answer 6

c

Question 7

Beef heart, Main antigen

a. Cardiolipin
b. Lecithin
c. Cholesterol
d. 1% Benzoic Acid
e. Buffer

Answer 7

a

Question 8

Preservative

a. Cardiolipin
b. Lecithin
c. Cholesterol
d. 1% Benzoic Acid
e. Buffer

Answer 8

d

Question 9

What is the pH of the buffer?

Answer 9

6 +/- 0.1

Question 10

What is the apperance of Ag suspencion?

Answer 10

milky and turbid

Question 11

Ag suspension are stored in ________ bottle because it is _______ sensitive

Answer 11

Amber

Sensitive

Question 12

What syringe is used for calibration or Testing of a Delivery needle?

Answer 12

Hamilton syringe

Question 13

What is the bevel for Hamilton syringe?

Answer 13

No bevel

Question 14

What is the needle gauge for:

VDRL (Qualitative)
Vol: 17 ul or 1/60ml
Drops: 60 +/- 2 drops per mL

Answer 14

18

Question 15

What is the needle gauge for:

VDRL (_________)
Vol: 14 ul or 1/75ml
Drops: 75 +/- 2 drops per mL

Answer 15

19

Quantitative

Question 16

What is the needle gauge for:

VDRL (_________)
Vol: 10 ul or 1/100ml
Drops: 100 +/- 2 drops per mL

Answer 16

21 or 22

CSF

Question 17

What is the needle gauge for:

_____________
Vol: 17 or 16 ul; 1/60ml
Drops: 60 +/- 2 drops per mL

Answer 17

20

RPR

Question 18

What is the specimen for VDRL? Volume?

Answer 18

Serum (50uL)

Question 19

Inactivating the serum for VDRL:

56C for ______
_______ for 2-3 mins

Answer 19

30 mins

60-62

Question 20

What is the reason for heating the serum?

Answer 20

Inactivation of complement

Question 21

If Serum is inactivated for more than 4 hours previously, it should be re-inactivated for ___mins at ___ C

Answer 21

10

56

Question 22

CSF requires heating for complement

T or F

Answer 22

F

no heating since no complement

Question 23

CSF sample is for what complication in syphilis?

Answer 23

Neurosyphilis

Question 24

In CSF, if traumatic tap, Centrifuge and obtain supernatant

T or F

Answer 24

T

Question 25

Specimen used is CSF

a. VDRL slides
b. Boerner’s Slide

Answer 25

b

Question 26

Specimen used is serum

a. VDRL slides
b. Boerner’s Slide

Answer 26

a

Question 27

Test Ring: 14mm

a. VDRL slides
b. Boerner’s Slide

Answer 27

a

Question 28

Test ring is 16 mm

a. VDRL slides
b. Boerner’s Slide

Answer 28

b

Question 29

Depth: 1.75

a. VDRL slides
b. Boerner’s Slide

Answer 29

Both

Question 30

Serum VDRL test:

Rotate for ___ mins at ____rpm

Answer 30

4

180

Question 31

Serum VDRL test examines for flocculation under what Objective power?

Answer 31

LPO

Question 32

What is the working temp when examining flocculation in VDRL?

Answer 32

23-29 C or 75-85 F

Question 33

Patient has Systemic Lupus erythematosus , what happens in VDRL test?

Answer 33

False positive

Question 34

What are other complication may lead to False positive in VDRL

Answer 34

Rheumatic Fever
IM
Malaria
Preg
SLE

(SM RIP)