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MTAP 2 Serology > 6.6 Treponemal Tests > Flashcards

6.6 Treponemal Tests Flashcards

1
Q

Also known as Nelson and Mayer (Reference test)

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

a

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2
Q

Recommended confirmatory test for syphilis by CDC

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

b

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3
Q

Specimen for Treponema Pallidum Immobilization test?

A

Serum

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4
Q

Principle: Indirect Fluorescent antibody test

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

b

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5
Q

Principle: Indirect hemagglutination

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

c

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6
Q

Similar to TPHA that uses Glutaraldehyde fixed avian RBC

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

d

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7
Q

Uses Live treponemes from infected rabbits and Guinea pig

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

a

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8
Q

What is the strain of live treponemes in Treponema Pallidum Immobilization test?

a. Reiter Strains
b. Nichol’s Strain

A

a

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9
Q

What is the analyte determinant for FTA-ABS?

A

Ab

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10
Q

Specimen for FTA-ABS

A

Serum

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11
Q

Reagent:
- Treponemal antigen
- FITC Labelled AHG (Fluorescent Ab)
- FTA ABS test Sorbent

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microocapsule test for T. pallidum

A

b

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12
Q

What is the strain for Treponemal antigen in FTA-ABS

a. Reiter Strains
b. Nichol’s Strain

A

b

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13
Q

Which of the following includes washing?

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

A

b

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14
Q

Includes Control cells and Test cells

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

A

c

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15
Q

Uses Colored Gelatin particles with antigen

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

A

e

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16
Q

Uses a carrier Capsule

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

17
Q

Principle: Semi-quantitative Particle agglutination assay

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

18
Q

This is used for resolving inconclusive FTA-ABS results

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

19
Q

In TPHA/MHA-TP:
Reagents:

Control cells: Avian RBCs coated with Treponemal Antigen (_______ Strains)

Test Cells: _______ RBCs coated with Treponemal antigen (______ Strain)

A

Reiter

Avian

Nichol’s

20
Q

Process:
- Sample binds to you reagent (Ag-Ab)
- Washing is done to remove excess
- Add FITC labelled AHG that is specific to the antibody from serum

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

21
Q

Eliminates the instability of RBC in TPHA

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

22
Q

In Treponema Pallidum Immobilization test:

Results:
(____): >50% or more Immobilization
(____): 20-50%
(____): <20%

A

+

Doubtful

-

23
Q

In FTA-ABS:
Heterogenous test:
- (+): __________________

A

Fluorescence

24
Q

In TPHA/MHA:
Results:

(+) Total/Partial ______ mat of cells
(-) Formation of _____________

A

Agglutination

Cell Buttom with w/ or w/o small hole

25
Q

In TPHA/MHA:

What is the Remedy for Negative with a cell button formed?

A

Repeat test due to interfering substances

26
Q

In TPHA/MHA:

MHA-TP: Same with TPHA but uses formalinized tanned sRBC with T. pallidum

T or F

27
Q

FTA-ABS is more sensitive than MHA-TP

T or F

28
Q

Uses positive and Negative control

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

29
Q

In Microcapsule test for T. pallidum,

What is the carrier capsule being used?

a. Urea
b. Polyurea
c. Uric acid
d. Latex

30
Q

What is the first serologic test for Syphilis

A

Wasserman Test

31
Q

Evolution of TPHA

a. Treponema Pallidum Immobilization test
b. Fluorescent Treponema Pallidum Antibody Adsorption Test (FTA-ABS)
c. TPHA/MHA-TP
d. HATTS (Hemagglutination Treponemal Test)
e. TP-PA (Treponema Pallidum Particle Agglutination
f. Microcapsule test for T. pallidum

MTAP 2 Serology (29 decks)

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