2.3 Immunoelectrophoresis Flashcards
In immunoelectrophoresis:
is a single-diffusion techniques that incorporates electricity to enhance result
T or F
F
Double diffusion
In immunoelectrophoresis:
Serum: Source of?
Ag
In immunoelectrophoresis:
Trough: place the?
Ab (Antisera)
In immunoelectrophoresis:
Double diffusion occurs at ______ angles
right
In immunoelectrophoresis:
Expected result?
Precipitin line / arc
In immunoelectrophoresis:
Qualitative or Quantitative?
Quali
In immunoelectrophoresis:
Procedures:
Prepare agarose gel
Antigens migrate under an electric current
Reagent Ab is added in the _______:Diffusion through gel
Formation of Precipitin Arc
Ab
Counter immunoelectrophoresis:
is a double immunodiffusion with electricity and a pH of _____ of the medium
8.6
Counter immunoelectrophoresis:
Antigen and antibody placed?
Parallel to each other (On one side and the other side)
Counter immunoelectrophoresis:
Is a 2 dimesion
T or F
F
1 dimension
Counter immunoelectrophoresis:
End result?
Precipitin line
Counter immunoelectrophoresis:
Rapid and sensitive
T or F
F
Not sensitive
Counter immunoelectrophoresis:
Quali or Quanti?
Quali
Rocket Electrophoresis:
is also known as?
Laurell electrophoresis
Rocket immunoelectrophoresis:
__________ is incorporated in the Agarose and the unknown ________ is placed on the well and electrophoresed
Antiserum
Antigen
Rocket immunoelectrophoresis:
The height of the rocket measured from the well to the apex and is indirectly proportional to the amount of Ag
T or F
F
Directly
Rocket immunoelectrophoresis:
Quali or Quanti?
Quanti
Rocket immunoelectrophoresis:
Is a Single immunodiffusion
T or F
T
Rocket immunoelectrophoresis:
Is 1 dimension
T or F
T
Immunofixation electrophoresis :
First described by?
Alper and Johnson
Immunofixation electrophoresis :
2 Stage Technique:
Use to separate the proteins (Ag)
Electrophoresis
Immunofixation electrophoresis :
2 Stage Technique:
Similar o immunoelectrophoresis except that antiserum is applied Directly rather than placed in the trough
Immunoprecipitation
Immunofixation electrophoresis :
Agarose and cellulose acetate can be used
T or F
T
Immunofixation electrophoresis :
Example?
Western, Southern, and Northern Blotting
Immunofixation electrophoresis :
Procedures:
Aliquots of the patient’s serum placed in each well
Perform electrophoresis: Separation of ____
Ag
Immunofixation electrophoresis :
In western blotting: What is being detected?
Antibody
