3.2 Types of Agglutination Flashcards
Type of agglutination:
Antigen is found naturally on the cell and contains no carrier
Direct agglutination
Type of agglutination:
ABO typing, Heme Agglutination
Direct agglutination
Type of agglutination:
Antigen is attached to carrier particles
Passive/Indirect Agglutination
Type of agglutination:
Widal test
Direct agglutination
Type of agglutination:
Example of carrier particles?
Latex
Charcoal
Type of agglutination:
Carrier particle includes RBC and Bentonite
T or F
T
Type of agglutination:
In PAssive, i RBC is used as Carrier what is the positive result?
Heme Agglutination
Type of agglutination:
Antibody is attached to the carrier particle
Reverse passive agglutination
Type of agglutination:
In Reverse passive agglutination, what is being detected?
Ag
Type of agglutination:
In reverse passive agglutination, what is the application for this?
CRP
Type of agglutination:
CRP is also known as?
Slide Latex test
Type of agglutination:
A 2 step process where homologous Ag inhibits agglutination of Ag coated particle
Agglutination inhibition
What is the specimen used for Agglutination inhibition?
Ag
Type of agglutination:
In Agglutination inhibition, the 1st step contains Insoluble Ag in px sample + Known Ab reagent
T or F
F
Soluble
Type of agglutination:
In Agglutination inhibition, the 2nd step is particulate Ag is added
T or F
T
Type of agglutination:
In Agglutination inhibition, What is the positive result?
No agglutination
Type of agglutination:
Antiglobulin techniques is also known as?
Coombs Test
Type of agglutination:
In Antiglobulin techniques, what type of Antibody is being detected?
Unexpected IgG
Type of agglutination:
In Antiglobulin techniques, Combs is added to bridge the gap between the cells
T or F
T
Composition of Coomb’s reagent?
Anti IgG; anti C3d
Type of agglutination:
In Antiglobulin techniques, What is the color of Coomb’s reagent?
Green
Type of agglutination:
In Antiglobulin techniques, Detects Sensitization
T or F
T
Type of agglutination:
In Antiglobulin techniques, to demonstrates incomplete antibodies
T or F
T
Type of agglutination:
In Antiglobulin techniques, a type of coombs involves in vivo sensitization
Direct Coombs
Type of agglutination:
In Antiglobulin techniques, a type of coombs involves in vitro sensitization
Indirect Coombs
Type of agglutination:
In Antiglobulin techniques, a type of coombs involves incubation to 37C before adding Coomb’s reagent
Indirect Coombs
Type of agglutination:
In Antiglobulin techniques, what is the sample for Indirect Coomb’s
Serum (Antibodies)
Type of agglutination:
In Antiglobulin techniques, What is the sample for Direct Coombs?
RBC
Type of agglutination:
The carrier particle used in this type of agglutination is bacteria
Coagglutination
Type of agglutination:
In Coagglutination, what bacteria is frequently used?
S. aureus
Type of agglutination:
In Coagglutination, what strain in S. aureus contributes in coagglutination
Cowan strain
Type of agglutination:
In Coagglutination, Staph aureus has a surface protein called _______ which naturally adsorbs the Fc portion of Ab
PRotein A
Type of agglutination:
This exhibits greater stability than latex particles and are more refractory to change in ionic strength
Coagglutination
