5.3 Classification of Indicator Labeled Immunoassays Flashcards
Principle of this is labeled and unlabeled analytes can compete for the same site on the antibody
a. Competitive
b. Noncompetitive
a
In competitive immunoassay, the measured signal is inversely correlated to the concentrations of analyte in the sample
T or F
T
In competitive, the higher the signal, the lower the antigen concentration
T or F
T
NOTE: Label produces the signal in competitive
For Competitive, this includes Radioimmunoassay
T or F
T
A highly specific and sensitive assay that involves the use of radioisotopes as it label or marker
Radioimmunoassay
Radioimmunoassay, What is the means to measure the analyte?
Scintillation counter (Gamma COunter)
This employs enzymes as the label for competitive immunoassay
EMIT (Enzyme multiplied immunoassay technique)
EMIT usually used Heterogeneous format
T or F
F
Homogeneous format
The application of EMIT is the detection of small molecules such as Hormone, especially _____
Drugs (Board exam)
Application for this is to detect small molecules such as Drugs
EMIT
Speciment for drug testing using EMIT?
Urine
Advantage of EMIT?
No washing
The higher signal for EMIT, The lower amount for drugs
T or F
T
A non classical 2 stage competitive immunoassay that can be used to detect illegal and therapeutic drugs
FPIA (Fluorescence polarization immunoassay
Specimen for FPIA when it comes to drugs
Urine
In FPIA:
(+) = Slower / decreased polarization
(-) = Faster / Increased polarization
T or F
T
Detection of either Ag or Ab in biological sample
a. Competitive
b. Non competitive
b
Ag and Ab are permitted to interact without deliberate introduction of competing molecules
Non compettive
Most commonly used immunoassay in noncompetitive?
ELISA
Non-competitive:
Method uses a labeled primary antibody that reacts directly with the Ag
a. Direct detection
b. Indirect detection
a
Direct Elisa is a heterogeneous assay
T or F
T
Direct elisa contains washing
T or F
T
In Direct Elisa, If the antigen is bound to the assay, is the primary antibody labeled?
yes
Application for Direct Detection?
Immunohistochemistry
Non-competitive:
Method is the most popular for ELISA
a. Direct detection
b. Indirect detection
b
Non-competitive:
More sensitive
a. Direct detection
b. Indirect detection
b
In indirect detection, if the antigen is bound to the assay, the primary antibody is unlabeled or labeled?
Unlabeled
In indirect detection, after the primary antibody is bound to the bound antigen, the secondary antibody is added which is labeled or unlabeled?
Labeled
In indirect detection, The secondary antibody is an anti _____ that binds to the ___ portion of the antibody
IgG
Fc
Non-competitive:
Has strong signal that even the smallest amount can be detected
a. Direct detection
b. Indirect detection
b
This assay is sensitive, Robust that is in ELISA assay format where analyte (To be measured bound between 2 antibodies which are capture Ab;Detector Ab
Sandwich assay
Ab source for Sandwich assay?
Rabbit and Mice
