2.2 Types of Precipitation reactions Flashcards
Precipitation in liquid:
Test tube method: Tubidimetry
Slide method: Use microscope
T or F
T
Precipitation in liquid:
Capillary method: Dense granulation in the metaphase
T or F
F
Interphase
Precipitation in liquid:
Ring test: Precipitin ____ formation
Ring
Precipitation in liquid:
Example of Ring test?
Ascoli Test
Precipitation in liquid:
Where do Ascoli test is used?
Anthrax (B. anthrasis)
Precipitation in Semi solid Medium contains?
1% Agarose
Single immunodiffusion: 1 object Movement
Single Dimension: 1 directions
T or F
T
Single immunodiffusion, Single dimension is also known as?
Oudin test
Single immunodiffusion, Single dimension:
PAtient sample with ________ and the agar is impregnated with _________
Ag
Antisera (Ab)
Single immunodiffusion, Single dimension:
(+) result?
Precipitin bond/ring
Single immunodiffusion, Single dimension:
Only antigen moves
T or F
T
Double immunodiffusion: 2 objects move
T or F
T
Double diffusion in One Dimension is also known as?
Oakley Fulthorpe method
Double diffusion in One Dimension:
_____ is incorporated in agar, poured into a tube and allowed to harden
Ab
Double diffusion in One Dimension:
A second layer of agar without ________ is placed above and allowed to solidify
antibody
Double diffusion in One Dimension:
___________ is placed above the agar (The very top)
Sx-Ag
Double diffusion in One Dimension:
(+) Result: The precipitin band appears in the?
Agar without the antibody
Immunodiffusion in 2 dimension has 2 or more movement
T or F
T
Immunodiffusion in two dimensions is usually plated
T or F
T
Single diffusion, Double Dimension is also known as
Radial immunodiffusion
Single diffusion, Double Dimension: Uses a plate containing agar with?
Ab
Single diffusion, Double Dimension:
___________ is placed on the wells
Ag
Single diffusion, Double Dimension:
The diameter of the precipitin is ___________ proportional to the concentration of the target antigen
Directly
Single diffusion, Double Dimension:
Antigen moves toward the agar gel
T or F
T
Single diffusion, Double Dimension:
Methods:
Also known as the end point method where the square of the diameter is directly proportional to the conc. of Ag
Mancini
Mancini usually takes?
24-72 hrs
Single diffusion, Double Dimension:
Method:
A kinetic method where the diameter is proportional to the log of the concentration of Ag
Fahey Mckelvey
Fahey Mckelvey usually takes?
18 hrs
Double diffusion, double dimension is also known as?
Ouchterlony or Double Angular immunodiffusion
Double diffusion, double dimension:
is also in the plated media
T or F
T
Double diffusion, double dimension:
Diffusion of both?
Ag / Ab
Double diffusion, double dimension:
Diffusion of 3 reagents placed in wells (___ Ag and ___ Ab)
2 and 1
Double diffusion, double dimension:
Diffusion in all directions
T or F
T
Double diffusion, double dimension:
This test can be used to find our the relationship between antibody
T or F
F
Antigen
Double diffusion, double dimension:
Has a complicated diffusion patterns
T or F
T
Double diffusion, double dimension:
This result forms a perfect precipitin arc
Identity
Antigens is very identical
Double diffusion, double dimension:
This result forms a cross instead of Arc
Non identity
Double diffusion, double dimension:
This result forms a spur
PArtial identity
Antigens are identical but not the same
