8 – Female Reproduction III Flashcards
1
Q
Estrous cycle
A
- Adjective
- Period between one estrus and the next
1
Q
Estrus
A
- Noun
- Referred to as heat
2
Q
Monovular
A
- Single oocyte per estrous cycle
3
Q
Polyovular
A
- More than one oocyte per estrous cycle
4
Q
Monotocous
A
- Produce one offspring
5
Q
Polytocous
A
- Produce more than one offspring
6
Q
What 2 cycles are in a mammals reproductive cycle?
A
- Estrous cycle
- Menstrual cycle
7
Q
What are the primary behavioural events during the estrous cycle?
A
- Sexual receptivity
- Copulation
8
Q
If fertilization does NOT occur during estrous cycle
A
- Another estrous cycle begins
- Provides female another opportunity to mate and conceive
9
Q
If fertilization DOES occur during estrous cycle
A
- Enter a period of anestrus that ends after:
o Parturition
o Uterine involution (normal uterus size and function)
o Lactation
10
Q
What are 3 types of estrous cyclicity?
A
- Polyestrous
- Seasonally polyestrous
- Monoestrous
11
Q
Polyestrous
A
- Uniform distribution of estrous cycles that occur REGULARLY throughout the entire year
- Ex. cow, pig, rodents
12
Q
Seasonally polyestrous
A
- Display ‘clusters’ of estrous cycles that occur ONLY during certain SEASON of the year
- Ex. horses (spring and summer: longer days)
- Ex. sheep, elk (winter: shorter days)
13
Q
Monoestrous
A
- Only one cycle per year
- Ex. dog, wolf, bear
14
Q
2 distinct phases of estrous cycle
A
- Follicular phase
- Luteal phase
*named after dominant structure present on ovary during each phase
15
Q
Follicular phase
A
- Regression of CL to ovulation
- ~20% of estrous cycle
16
Q
Follicles
A
- Dominant ovarian structure
- Produces estrogen=dominant hormone
- Products are E2, inhibin a+b=most important suppressors of FSH
17
Q
Follicular phase is subdivided into
A
- Proestrus stage
- Estrus stage
18
Q
Proestrus stage
A
- Formation of ovulatory follicles + E2 secretion
- 2-5 days
19
Q
Estrus stage
A
- Sexual receptivity + peak of E2 secretion
20
Q
Luteal phase
A
- Ovulation until CL regression
- ~80% of estrous cycle
21
Q
CL
A
- Produces progesterone=dominant hormone=suppressor of LH
22
Q
Luteal phase is subdivided into
A
- Metestrus
- Diestrus
23
Q
Metestrus stage
A
- CL formation + beginning of P4 secretion
- 2-5 days
24
Diestrus stage
* Sustained luteal secretion of P4
* 10-14 days
25
When are females showing sexual behaviour and why?
* Estrus stage
* Due to estradiol
26
Estrous cycle in pig
* Proestrus, estrus, metestrus, diestrus
* Polyovular (15-30 ovulations per cycle)
* Polyestrous
* Follicular waves not documented
* Luteolysis: day 15-18 (CL resistant to PGF2alpha until day 12)
27
Estrous cycle in bison
* Calves born in SYNCHRONOUS pattern in wild (seasonal reproductive pattern)
* Ovulatory season: late July to October
* Wave emergence linked to FSH stage
* Estrous cycle is 21days (estrus lasts 9-28hrs)
* Continuous emergence of anovulatory waves during anovulatory season (Feb-July)
28
Estrous cycle in Wapiti
* Seasonally polyestrous (SHORT day breeder=winter)
* 2-4 follicular waves
* Estrous cycle: 18-21 days
29
Estrous Cycle in mares
* Seasonally polyestrous (LONG day breeder=spring)
* Estrous cycle: 21 days
* ~2 follicular waves
* Estrus behaviour ceases: 24-48hrs after ovulation
* If not pregnant: luteolysis day 14-16
* Anestrus in winter months (seasonal anestrus)
30
Estrus length in mares
* Estrus: 6 days (4-10days)
31
What signals the start of the season in mares?
* Production of GnRH
* Stimulates A pituitary
* A. pituitary produces FSH and LH
32
Long vs short day breeders (seasonal polyestrous)
* Relies on MELATONIN
* Winter: increased melatonin
o Stimulates GnRH in short day breeders=cyclicity
o Inhibits GnRH in long day breeders=anestrus
*OPPOSITE FOR IN THE SPRING/SUMMER
33
Estrous cycle in dogs: stages
* Proestrus, estrus, diestrus, anestrus (no metestrus)
34
Estrous cycle in dogs
* Polytocous
* Monoestrous
* Seasonal (Feb-May)
* Follicular waves not document
* Luteal phase similar between pregnant and non-pregnant (pseudopregnancy)
o Don’t use progesterone to test pregnancy
35
Why is estrus so long in dogs?
* 9 days
* Nature knows they will come into heat only once a year=making sure they have more than enough time to do mating
36
Why is fertilization delayed in dogs and horses?
* Follicle will release primary oocyte and become secondary oocyte later
o Primary oocyte=can NOT be fertilized
* *needs time to change
37
Estrous cycle in cats: stages
* Proestrus
* Estrus
* Post-estrus
* Diestrus
* Anestrus
38
Post estrous
* Describes inter-estrus period that follows estrus that has NOT been induced to ovulate by copulation
* If follicle has not left oocyte=wont go into luteal phase
39
Estrous cycle in cats
* Seasonally polyestrous
* Induced ovulatory (need external stimuli)
* Follicular waves not documented
* Luteal phase: 60days in pregnant, 40days in non-pregnant (pseudopregnant)
* Proestrus+estrus: 2-20 days
* Anestrus (if mated): 30-90 days
40
Types of anestrus
1. Gestational
2. Seasonal
3. Lactational
4. Nutritional
5. Pathological
41
Lactational anestrus
* Limits pregnancy until young are weaned
* Variable among species (none in mares and camelids)
42
Lactational anestrus: sows
* Ovulate 6-8 days after weaning
43
Lactational anestrus: dairy vs. beef
* Distinct effect of suckling of her OWN calf
* Prevent suckling: acute increase LH pulse frequency (hastened ovulatory cyclicity)
44
Lactational anestrus: mares
* ‘foal heat refers to first estrous cycle that occurs in a mare shortly after giving birth, 6-8 days pp (first ovulation ~10days)
45
Lactational anestrus: camelids
* Induced ovulators
* Receptive immediately postpartum, but no ovulation until after 14days
46
Lactational anestrus: women
* Resume menstrual cycles after 8-12 months if not lactating
* Beyond 24months if nursing
*energy balance is influential
47
Nutritional anestrous
* Absence of GnRH pulses from hypothalamus=inadequate secretion of gonadotropins and inactive ovaries
* ENERGY demands different between primiparous vs. multiparous
* Feed quality and quantity (energy-rich supplements)
48
Primiparous
* First time birthing
* Higher energy demand
49
Multiparous
* Having had multiple offspring
* Lower energy demand
50
Menses (menstration)
* Sloughing of endometrium to exterior
51
How does the menstrual cycle differ from the estrous cycle?
* No defined period of sexual receptivity
* Period of endometrial sloughing (=menses)
* Timeline begins and ends with menses (not ovulation or estrus)
* Ovulation occurs at day 14, rather than beginning and end of cycle
52
Manipulation of ovarian function: depends on status of CL and follicular wave (differs between 2- and 3-wave patterns)
1. Shorten luteal function: treat with PGF2alpha (=regresses CL)
2. Lengthen luteal phase: treat with P4
53
What is ovarian synchronization for?
* Fixed-time insemination
54
Synchronized wave emergence
* Follicle ablation
* Estrogen and progesterone
* GnRH/LH
55
Follicle ablation for ovarian synchronization
* Put ultrasound in and remove dominant follicle
o Now animal will start a new follicular wave (1.5 days later)
56
Estradiol and progesterone for ovarian synchronization
* CIDR (Controlled internal drug release)
o Release progesterone
o Once remove=new follicular wave in 4 days
57
GnRH for ovarian synchronization
* Will release oocyte if close to development
* New follicular wave will begin
* *only true up to 60% in most cattle
58
What is ovarian super stimulation used for?
* in vivo and in vitro embryo production and embryo transfer
* Controlled breeding management
59
Superstimulation
* Induce development of multiple follicles
o Release of multiple oocytes (eggs) during a SINGLE estrous cycle
* More effective if do it before selection of dominant follicle
* Only 20% of cycle is available for initiating treatment at time of follicular wave emergence
60
Ovarian superstimulation: extrinsic factors
* Gonadotrophins
* Purity
* Dose
* Route
* Frequency
* Site
61
Ovarian superstimulation: intrinsic factors
* Nutritional status
* Reproductive history
* Age
* Season
* Breed
* *ovarian status