6 – Female Reproduction I Flashcards

1
Q

Ovary function

A
  • Development of female gamete (oocyte) for fertilization
  • Produce and secret sex steroid hormones to regulate reproductive health
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2
Q

Ovary divided into 2 distinct regions

A
  • Outer cortex
    o Ovarian follicles
  • Inner medulla
    o Blood vessels
    o Nerves
    o Connective tissue
  • *opposite orientation in mares but contents are the same
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3
Q

Folliculogenesis

A
  • Development of ovarian follicles from immature to mature form
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4
Q

2 stages of folliculogenesis

A
  1. Resting phase: primordial follicles
  2. Growth phase
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4
Q

Folliculognesis stages

A
  • Primordial
  • Primary
  • Early secondary
  • Secondary
  • Antral (early and late)
  • Preovulatory
    o Ovulation OR follicular death by atresia
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5
Q

Primordial follicles

A
  • Specific number at birth
  • Primary oocyte surrounded by a single layer of flat follicular cells
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6
Q

Ruminant primordial follicle

A
  • Equally distributed in cortex
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7
Q

Carnivores primordial follicle

A
  • In clusters in the cortex
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8
Q

Bipotential gonad

A
  • Development of a testis OR ovary
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9
Q

3 cell populations during gonad transformation to ovaries

A
  1. Granulosa cells
  2. Theca cells
  3. Primordial germ cells
    *3 components of the follicle
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10
Q

Granulosa cells

A
  • Supporting cells
  • Subtype of somatic cells
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11
Q

Theca cells

A
  • Steroidogenic cell precursors
  • Subtype of somatic cells
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12
Q

Primordial germ cells

A
  • Become oocytes through the OOGENESIS
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13
Q

Where does the ovary development commence?

A
  • Mesonephric surface epithelium in location of future gonadal ridge
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14
Q

Primary follicle

A
  • When epithelium around primary oocyte becomes cuboidal
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15
Q

Secondary follicle

A
  • 2-6 layers of granulosa cells
  • Theca cells
  • Zona pellucida material
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16
Q

Theca cells

A
  • Typical steroid-producing cells
  • Additional somatic cell layer
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17
Q

Zona pellucida

A
  • Glycoprotein layer secreted by the oocyte and granulosa cells
18
Q

Tertiary (antral) follicle

A
  • More than 6 layers of granulosa cells
  • Have an antrum
  • Fully developed ZP
  • External and internal theca cell layers
  • Start of cumulus cell formation
19
Q

Antrum

A
  • Fluid (follicular)-filled cavity
  • Fluid comes from granulosa and theca cells
20
Q

Graafian follicles (pre-ovulatory)

A
  • Most mature of antral follicles (still is an antral follicle)
  • Granulosa cells produce estradiol
  • Internal theca=vascular
  • External theca=connective tissue
21
Q

Graafian follicles: zona pellucida

A
  • Surrounds oocyte
  • Located between oocyte and follicular cells
22
Q

Graafian follicles: corona radiata

A
  • First layer of follicular (granulosa) cells outside the ZP
23
Q

Graafian follicles: cumulus oophorus

A
  • ‘egg-bearing’ heap
  • Thickened mound of granulosa cells that surround oocyte and projects into antrum
24
Q

Graafian follicles: membrane granulosa

A
  • Layer of granulosa cells bordering the antrum
25
Q

Graafian follicles: theca interna

A
  • Endocrine-like
  • Border the membrane granulosa
26
Q

Graafian follicles: theca externa

A
  • Fibroblast-resembling cells just outside theca interna
  • Exterior limits of it are NOT discernible
27
Q

Atretic (regressing) follicles

A
  • Can occur at any follicular phase
  • ACTIVE cellular process with resorption of follicle
  • NOT all Graafian follicles ovulate
28
Q

ACTIVE cellular process with resorption of follicle involving

A
  • Macrophage infiltration
  • Phagocytosis
  • Migration of fibroblasts from theca
  • Production of collagen
29
Q

2 different types of ovulation

A
  • Spontaneous: most domestic animals=NEED external stimuli to ovulate
  • Induced: queen, camelids, rabbit
30
Q

What happens near ovulation?

A
  • Blood supply increases
  • Follicular wall becomes thin and transparent near future site of ovulation
31
Q

Size of preovulatory follicles: mare

A
  • 50-70mm
32
Q

Size of preovulatory follicles: cow

A
  • 13-16mm
33
Q

Size of preovulatory follicles: ewe, goat, sow

A
  • 8-10mm
34
Q

Size of preovulatory follicles: dog and queen

A
  • 2mm
35
Q

Stigma

A
  • Site where follicle ovulates
36
Q

Oocyte development

A
  • PGCs at gonadal ridges differentiate into oogonia
  • Oogonia enter meiosis I (primary oocytes)
  • Primary oocytes initiate meiosis but are arrested in diplotene stage or prophase I
  • Primary oocyte completes first meiotic division immediately before ovulation to form secondary oocyte and first polar body
  • First division completed shortly after ovulation in dog and mare
37
Q

Corpus luteum (CL)

A
  • Transient endocrine gland
  • Develops from a Graafian follicle after ovulation
  • Required to support pregnancy in mammals
    o Produces progesterone
38
Q

Corpus hemorrhagicum

A
  • When ovulation of Graafian follicle has occurred=blood derived from torn blood vesicles of the theca layer infiltrates the collapse follicle
  • Developing CL with a bloody core
39
Q

CL: remaining granulosa cells and cells of theca interna undergo

A
  • Hypertrophy, proliferate and invade corpus hemorrhagicum
  • Transforms into granulosa lutein cells and theca lutein cells =CL
    o Yellow due to lutein or other lipid pigments
40
Q

CL in cows

A
  • Fully developed by 9days after ovulation
  • Continues to grow till 12 days
  • Regresses in late diestrus
41
Q

What happens to CL when pregnancy does NOT occur?

A
  • CL degenerates after a certain length of time (species dependant)
  • Condensation of lutein pigment and degeneration of luteal cells
  • Becomes corpus albicans
42
Q

Corpus albicans

A
  • Connective tissue that replaces the luteal cells from the regressed CL
  • *WHITE