7th-Biochemistry-Exam-DNA-and-RNA-Metabolism-Batch-2025 COPY Flashcards
1.True about the non-template strand except:
A. Strand that is transcribed
B. Also termed coding strand
C. Corresponds exactly to the sequence of RNA primary transcript except T for U
D. None of the above
A. Strand that is transcribed
2.The product of DNA transcription is called:
A. Mature mRNA
B. Introns
C. Primary transcript
D. Exons
C. Primary transcript
- Nonprotein coding RNA that participates in mRNA splicing:
A. miRNA
B. siRNA
C. snRNA
D. lncRNA
C. snRNA
4.The most abundant of all types of RNA comprising about 80% of the total:
A. mRNA
B. tRNA
C. snRNA
D. rRNA
D. rRNA
5.Similarities of DNA and RNA synthesis include the following except:
A. The general steps of initiation, elongation and termination with 5’ to 3’ polarity
B. Large multicomponent initiation complexes
C. Both have highly active efficient proofreading function
D. Adherence to Watson-Crick base-pairing principles
C. Both have highly active efficient proofreading function
6.This includes the promoter, RNA-coding region and a terminator:
A. Transcription unit
B. Primary transcript
C. 5’ Flanking sequences
D. 3’ Flanking sequences
A. Transcription unit
7.The RNA polymerase that has intermediate sensitivity to alpha-amanitin:
A. Pol I
B. Pol II
C. Pol III
D. Pol IV
C. Pol III
8.The major product/products of RNA Polymerase I is:
A. rRNA
B. mRNA, lncRNA,miRNA,snRNA
C. tRNA, 5s RNA
D. 45S RNA
A. rRNA
9.RNA polymerase attaches to this site to start the initiation process:
A. Guanosine triphosphate cap
B. Enhancer
C. Primer
D. Promoter
D. Promoter
10.All nucleotides upstream of the site of transcription termination are termed:
A. 5’ Flanking sequences
B. 3’ Flanking sequences
C. Pre-initiation complex
D. Closed complex
A. 5’ Flanking sequences
11.The type of RNA polymerase that catalyzes the synthesis of mRNA:
A. Pol I
B. Pol II
C. Pol III
D. Pol IV
B. Pol II
12.The TATA box in eukaryotes is located:
A. 10 bp upstream from the transcription start site (TSS)
B. 25-30 bp upstream from TSS
C. 35 bp upstream from the TSS
D. 5 bp upstream from the TSS
B. 25-30 bp upstream from TSS
13.The termination protein is also called:
A. Psi factor
B. Sigma factor
C. Alpha factor
D. Rho factor
D. Rho factor
14.In some mRNA encoding genes that lack a consensus TATA box, this directs the RNAP
A. Downstream promoter element
B. Enhancers
C. General transcription factors
D. Primer
A. Downstream promoter element
15.The general transcription factor (GTF) that binds to the TATA box promoter element:
A. TFIIA
B. TFIIB
C. TFIIC
D. TFIID
D. TFIID
16.Phosphorylation of Pol II results to:
A. Increased activity
B. Reduced activity
C. No change
D. Static activity
A. Increased activity
17.On certain eukaryotic genes, transcription machinery cannot access the promoter because they are wrapped in:
A. Spliceosomes
B. Ribosomes
C. Nucleosomes
D. Ribozymes
C. Nucleosomes
18.Processing of mRNA primarily occurs in the:
A. Cytoplasm
B. Ribosomes
C. Nucleus
D. Nucleolus
C. Nucleus
19.The RNA sequence that appears in mature RNAs:
A. Exons
B. Introns
C. Cap
D. Tail
A. Exons
20.Intervening sequences of DNA that neither appear in mature mRNA nor contribute to the genetic information:
A. Exons
B. Introns
C. Cap
D. Tail
B. Introns
21.Removal of intervening sequences and splicing together of final sequences are done by:
A. Spliceosome
B. Nucleosome
C. Ribosome
D. None
A. Spliceosome
22.In mammalian cells, the three rRNA molecules are transcribed as part of this single large precursor molecule:
A. 40S
B. 32S
C. 45S
D. 24S
C. 45S
23.The precursor rRNA molecule is processed in the:
A. Nucleus
B. Nucleolus
C. Ribosome
D. Mitochondria
B. Nucleolus
24.At the 5’end, mRNA molecules are capped with:
A. Poly (A) tail
B. TATA cap
C. 7-methylguanosine cap
D. GCCT tail
C. 7-methylguanosine cap