2nd Biochemistry Lecture Exam (Batch 2026) Flashcards
1.ELISA was developed from modification of RIA. This method was first employed to determine?
A. Thyroid levels in blood
B. HCG in urine
C. FSH in breast milk
D. Influenza in saliva
B. HCG in urine
2.This type of ELISA utilizes two specific antibodies; an enzyme conjugated antibody and an antibody in the serum?
A. Direct
B. Indirect
C. Sandwich
D. Competitive
D. Competitive
3.This type of ELISA utilizes an antigen coated-plate and a screening antigen or antibody?
A. Direct
B. Indirect
C. Sandwich
D. Competitive
B. Indirect
4.Which of the following factors may interfere with the antibody capture and detection resulting to inappropriate ELISA testing?
A. Material of the plate
B. Cross reactivity
C. Titer and affinity
D. Conformation, stability
C. Titer and affinity
5.The result and reporting that will deny or confirm the presence of a particular antigen/ antibody in a sample?
A. Quantitative
B. Qualitative
C. Semiqualitative
D. Semiquantitative
B. Qualitative
6.The use of Aspariginase in patients with ALL may be limited due to its toxicity that may be severe?
A. Hypersensitivity reaction
B. Anaphylaxis
C. Pancreatitis
D. Coagulation abnormalities
B. Anaphylaxis
7.During the treatment with Aspariginase, malignant cells are unable to produce asparagine due to?
A. High number of destroyed cells
B. Increase amount of substrate
C. Lack of synthase
D. Inhibition from normal cells
C. Lack of synthase
8.Newer asparaginase of which the toxicity is reduced are derived from?
A. E. coli
B. E. chrysantemi
C. E. elspar
D. E. histolytica
B. E. chrysantemi
9.Tripsinogen are members of the pancreatic enzymes whos function are?
A. Proteolytic
B. Amylolitic
C. Lipolitic
D. Hydrolitic
A. Proteolytic
10.Efficacy of endogenous pancreatic enzymes are reduced by?
A. Low pH
B. Bicarbonates
C. Small intestinal secretion
D. Esophageal actions
A. Low pH
11.In determining the absolute quantity of enzyme, which of the following factors will increase the amount of enzyme?
A. ↑ rate of synthesis, ↓ rate of degradation
B. ↑ rate of synthesis, ↑ rate of degradation
C. ↓ rate of synthesis, ↓ rate of degradation
D. ↓ rate of synthesis, ↑ rate of degradation
A. ↑ rate of synthesis, ↓ rate of degradation
- Kinases require binding to Magnesium (Mg+) ion. Magnesium is an example of:
A. Co-factor
B. Co-enzyme
C. Co-substrate
D. Co-repressor
A. Co-factor
13.Which of the following correctly represents the mechanism of enzyme function:
A. S+P → S-P → E-P → E+P
B. E+P → E-P → E-S → E+S
C. E+P→ E-S → E-P → E+P
D. E+S→ E-S → E-P → E+P
D. E+S→ E-S → E-P → E+P
14.Some enzymes are catalytic RNA molecules. These are called:
A. Apoenzymes
B. Holoenzymes
C. Ribozymes
D. Abzymes
C. Ribozymes
15.Antibodies that possess catalytic properties are called:
A. Apoenzymes
B. Holoenzymes
C. Ribozymes
D. Abzymes
D. Abzymes
16.The following enzymes are of diagnostic use for Myocardial infarction EXCEPT:
A. Acid phosphatase
B. Aminotransferase
C. Creatine Kinase
D. Lactate dehydrogenase
A. Acid phosphatase
17.Amylase and lipase are enzymes that can used to diagnose:
A. Viral hepatitis
B. Acute pancreatitis
C. Myocardial infarction
D. Wilson’s disease
B. Acute pancreatitis
18.Which of the following statements regarding enzymes is TRUE:
A. Enzymes are consumed during chemical reactions.
B. Without enzymes, metabolic reactions will not occur.
C. Enzymes catalyze only one type of chemical reaction
D. Enzymes selectively channel products into useful pathways
C. Enzymes catalyze only one type of chemical reaction
19.These enzymes are also known as dehydrogenases
A. Hydrolase
B. Lyase
C. Ligase
D. Oxido-reductase
D. Oxido-reductase
20.Digestive enzymes such as Amylase, Cellulase and Sucrase belong to this class of enzymes.
A. Hydrolase
B. Lyase
C. Ligase
D. Oxido-reductase
A. Hydrolase
21.This class of enzyme follows this typical reaction: AB + H2O → AOH +BH
A. Hydrolase
B. Lyase
C. Ligase
D. Oxido-reductase
A. Hydrolase
22.What class of enzymes catalyzes removal of a functional group from a substrate and cleavage of covalent bonds without the addition of water
A. Hydrolase
B. Lyase
C. Ligase
D. Oxido-reductase
B. Lyase
23.Glucose 6-phosphate is converted to Fructose 6-phosphate with an enzyme that belongs to which class of enzymes?
A. Transferase
B. Isomerase
C. Ligase
D. Oxido-reductase
B. Isomerase
24.Pyruvate carboxylase converts Pyruvate and CO2 to Oxaloacetate in the presence of ATP. Pyruvate carboxylase is classified as a/an:
A. Transferase
B. Isomerase
C. Ligase
D. Oxido-reductase
C. Ligase