7.2 kitchener's microscopes Flashcards
cytoarchitectonics is the classification of neural tissue on what basis?
number, type and density of neurons within a region
who defined 50 distinct regions of cortex and based on what?
Brodman, based on cellularity
how is microscopic imaging limited?
wavelength of light - maximum resolution is 250nm
what microscopic technique works at distances closer than one wavelength?
total internal reflection fluorescence (TIRF)
what is the best thickness that TIRF can deal with?
about 100nm of tissue thickness
Stimulated Emission Depletion (STED)…how does it work?
the point spread function of the diffraction disc (airy disc) is narrowed by using a laser to deactivate the outmost portion
Stochastic Optical Reconstruction (STORM)??
the random switching of fluorescence of individual molecules allows their individual contributions to be imaged, picture worked out from millions of locations
a high-resolution electrophysiology technique
patch-clamp electrodes
a technique with really good spatial and also temporal resolution
microelectrodes to record the activity of single neurons
