7. Lecture

Question 1

dsRNA

Answer 1

segmented genome
alien nucleic acid for the cell
→ intensive interferon response to these viruses released by host cells
→ partial decapsidation

Question 2

dsRNA vs. ssRNA transcription

Answer 2

•the dsRNA is not readable for the

ribosomes

Question 3

dsRNA Translation:

Answer 3

monocistronic coding:

mRNA contains the genetic information to translate only a single protein chain

Question 4

dsDNA: late transcription, translation

Answer 4

→structural protein synthesis for assembly of progeny virions

Question 5

dsDNA: early transcription, translation

Answer 5

→ protein synthesis for replication

Question 6

-ssRNA Translation

Answer 6

monocistronic

Question 7

Viruses using reverse transcriptase:

Answer 7

RNA/DNA
•Retroviridae
+ssRNA (diploid)

Question 8

Transcription of viruses using reverse transcriptase:

Answer 8

Transcription:
•cellular transcriptase: mRNA production
•long terminal repeat (LTR) regions
•intensive promoter
→ frequent transcription

Question 9

Genome of viruses using reverse transcriptase:

Answer 9

Genome ~ mRNA, but!:
•reverse transcriptase (structural)
•transcribes to dsDNA
The viral dsDNA integrates into the cellular
genome
→ provirus
→ cell division – virus genome copying

Question 10

translation of viruses using reverse transcriptase:

Answer 10

•polycistronic mRNA – protease cleavage

Question 11

replication of viruses using reverse transcriptase:

Answer 11

= Transcription!
•the mRNA will finally serves as the genome
of the progeny viruses