5.2 Study Guide Flashcards
Where does DNA replication take place in a Eukaryotic cell?
In the Nucleus
Where does DNA replication take place in a Prokaryotic cell?
In the Cytoplasm
When does DNA replication take place?
In interphase (Before Mitosis)
What are the 4(5) enzymes in DNA replication?
Helicase, DNA Polymerase (1 & 3), Primase, Ligase
What does DNA Polymerase (1 & 3) do in DNA replication?
“The Builder Enzyme”
3: Adds new nucleotides to build new DNA strands(only adds to existing strand)
1: Replaces the primer with real DNA to start the strand correctly.
What does Helicase do in DNA replication?
“The Unzipping Enzyme” Breaks the hydrogen bonds that hold DNA together.
What does Primase do in DNA replication?
Lays down RNA primers for DNA polymerase to start from.
What does Ligase do in DNA replication?
Attaches the fragments together, repairs breaks in the sugar-phosphate backbone
What are the two types of strands in DNA replication?
Leading strand & Lagging strand
What is the difference between a leading strand and a lagging strand?
Leading strand: Doesn’t have to stop adding
Lagging strand: Has to restart the process to continue.
Why is there a leading strand and a lagging strand at each replication fork?
The DNA Polymerase can only build from 5’ to 3’ direction, antiparallel.
What is the process of DNA replication?
Making new DNA before the cell is split.