5.2 Energy for biological processes Flashcards
why organisms require energy
active transport (e.g. endocytosis, sodium/potassium pump)
synthesis of large molecules e.g. protein
movement (brought about by cilia, flagella)
DNA replication
cell division
activation of molecules
how much energy released in hydrolysis of ATP
30.5 kJ mol^-1
hydrolysis of ATP
requires ATPases and water
ATP -> ADP + Pi
releases energy for cell to use
condensation of ATP
ADP + Pi -> ATP + H2O
requires energy generated from respiration
anabolic reaction definition
large molecules synthesised from smaller molecules
catabolic reaction definition
hydrolysis of larger molecules into smaller ones
ATP role
standard intermediary between energy-releasing and energy-consuming metabolic reactions
main storage of energy as releases energy in small amounts
hydrolysis is immediate and one step reaction so is quick
ATP structure
phosphorylated nucleotide
made up of adenine, ribose sugar, 3 phosphate groups
phosphodiester bond between ribose and phosphate group
phosphoanhydride bond between phosphate groups
why ATP is universal energy source
occurs in all living cells
source of energy that can be used in small amounts
energy definition
ability to do work
importance of hydrolysis of ATP and respiration releasing heat
keeps living organisms “warm”
helps maintain internal temperature for enzyme-controlled reactions
glycolysis summary
first stage in respiration
pyruvate is produced from glucose
occurs in cytoplasm
glycolysis stages
phosphorylation (1) (energy investment)
lysis
phosphorylation (2)
dehydrogenation and formation of ATP (energy generation)
phosphorylation (1)
energy investment phase
2 phosphate groups (released from 2 ATP molecules required)
attach to glucose molecule
forms hexose biphosphate
lysis in respiration
destabilises molecule
causes hexose biphosphate to split into x2 triose phosphate molecules
phosphorylation (2)
another phosphate group added to each triose phosphate
forms 2 triose biphosphate molecules
doesn’t require ATP as
phosphate groups come from free inorganic phosphate ions in cytoplasm
dehydrogenation and formation of ATP in glycolysis
two triose biphosphate molecules oxidised by dehydrogenase enzymes removing 1 hydrogen ATOM in each molecule(dehydrogenated)
forms 2 pyruvate molecules
NAD coenzymes accept removed hydrogen atoms (reduced), forms 2 NADH
4 ATP molecules formed from phosphate groups from triose biphosphate molecules
substrate level phosphorylation definition
formation of ATP without involvement of electron transport chain
alternate name for pyruvate
pyruvic acid
NAD stands for
nicotinamide adenine dinucleotide
NADH means
reduced NAD
what happens to pyruvate after glycolysis
actively transported into mitochondria for link reaction (aerobic conditions)
converted into lactate (anaerobic in animals)
converted into ethanol (anaerobic in plants and prokaryotes)
function of matrix in mitochondria
contains enzymes for Krebs cycle, link reaction, mitochondrial DNA
function of intermembrane space in mitochondria
proteins pumped in here by electron transport chain
conc. builds up quickly as space is small
function of outer mitochondrial membrane in mitochondria
separates contents of mitochondrion from rest of cell (compartmentalisation)
maintains ideal conditions for aerobic respiration
structures in inner mitochondrial membrane in mitochondria
contains electron transport chains and ATP synthase
function of cristae in mitochondria
projections of inner membrane increases surface area
faster rate of oxidative phosphorylation
why oxidative decarboxylation is called the link reaction
links anaerobic glycolysis to aerobic steps of respiration in mitochondria
link reaction steps
pyruvate enters mitochondrial matrix (actively transported by carrier protein pyruvate proton symplast)
pyruvate is decarboxylated (CO2 removed) and dehydrogenated (hydrogen atom removed)
NAD accepts hydrogen atoms (reduced to form NADH)
forms acetate (has acetyl group C=O)
acetyl groups bound by coenzyme A to form acetyl CoA
acetyl CoA in full
acetylcoenzyme A
link reaction as an equation
2pyruvate + 2NAD + 2CoA -> 2CO2 + 2NADH + 2 acetyl CoA
coenzyme A function
accepts acetyl group
forms acetyl CoA
carries acetyl group to Krebs cycle
Krebs cycle facts
occurs in mitochondria occurs twice per molecule of glucose forms 4 CO2 molecules (per molecule of glucose) forms 2 ATP forms 2 FADH2 forms 6 NADH
Krebs cycle method
acetyl group (delivered by acetyl CoA) combined with oxaloacetate to form citrate
citrate is decarboxylated and dehydrogenated, forms one NADH, one CO2 and 5 carbon compound
5 carbon compound decarboxylated and dehydrogenated further, eventually regenerating oxaloacetate
number of carbon on acetyl
2 carbons
number of carbons on oxaloacetate
4 carbons
number of carbons on citrate
6 carbons
how oxaloacetate is regenerated
5 carbon compound decarboxylated and dehydrogenated, forming one NADH, one CO2 and 4 carbon compound
4 carbon compound temporarily combines and released by CoA
substrate level phosphorylation occurs, 1 ATP made
4 carbon compound dehydrogenated, forming 1 FADH2 and different 4 carbon compound
atoms rearranged in 4 carbon molecule, catalysed by isomerase enzyme
dehydrogenated again (forms NADH), regenerates oxaloacetate
chemiosmosis definition
flow of protons down their concentration gradient across a membrane through a channel associated with ATP synthase
electron transport chain structure
chain of carrier transfer proteins containing Fe3+ ions
electron transport chain mechanism
NADH and FADH2 binds to complex I and reoxidised in matrix (releases hydrogen atoms as protons and electrons) to electron transport chain
hydrogen ions/protons enter solution in matrix
electrons pass along chain of electron carriers (complex 1 to 2 to 3 to 4) (Fe ions reduced (into 2+) and reoxidised (back to 3+))
energy created by electrons passing along chain used to pump protons across intermembrane space
how proton gradient is generated
protons accumulate in intermembrane space
proton gradient forms across membrane, generates chemiosmotic potential
what is chemiosmotic potential
also known as proton motive force (pmf)
source of potential energy
chemiosmosis mechanism
protons cannot diffuse through lipid bilayer (impermeable to protons as they are charged)
protons diffuse through proton channel associated with ATP synthase enzymes (in inner membrane) down proton concentration gradient
flow of protons cause conformational change in ATP synthase
causes ADP + Pi to combine to form ATP
purpose of oxygen in oxidative phosphorylation
combined with electrons coming off electron transport chain and with protons diffusing down ATP synthase channel
forms water
4H+ + 4e- + O2 -> 2H2O
how many protons pumped by complex I
4
how many protons pumped by complex II
none
how many protons pumped by complex III
4
how many protons pumped by complex IV
2
products of glycolysis
2 pyruvate
4 ATP (net = 2)
2 NADH