4C-The polymerase Chain reaction

Question 1

KEY TERM - Amplify

Answer 1

To increase the quanity of a molecule by making many copies

Question 2

KEY TERM - Anneal

Answer 2

Joining of two molecules, for example two complementry DNA strands during the cooling phase of PCR

Question 2

KEY TERM - Forward primer

Answer 2

A DNA primer that binds to the 3’ end of the Template strand and reads the DNA in the same direction as the RNA Polymerase

Question 3

KEY TERM - Reverse Primer

Answer 3

A DNA primer that binds to the 3’ end of the coding strand and reads the DNA in the reverse direction the the RNA polymerase

Question 3

KEY TERM - Polymerase Chain Reaction

Answer 3

A lab technique used to produce many identicle copies of DNA from a small initial sample

Question 4

KEY TERM - Taq Polymerase

Answer 4

A heat resistant DNA polymerase enzyme which amplifies single stranded DNA molecules by attaching complementry Nucleotides.

Question 5

Stage 1 of Polymerase chain reaction -

Answer 5

The DNA is heated to 90-95 degrees in order to break the hydrogen bonds between the twin strands

Question 6

Step 2 of Polymerase chain reaction -

Answer 6

Annealing stage - the DNA is then cooled to 50-55 degrees for the primers to the complementry stands

Question 7

Step 3 of Polymerase chain reaction -

Answer 7

Elongation stage - The DNA is once again heated to 72 degrees for the TAQ POLYMERASE to work optimally, synthesising the DNA.