4B-CRISPR-CAS 9

Question 1

KEY TERM - Bacteriophage

Answer 1

A virus that infects prokaryotic organisms

Question 2

KEY TERM - CRISPR-Cas9

Answer 2

A complex formed between gRNA and Cas9 which can cut a target sequence of DNA.

Question 2

KEY TERM - Cas9

Answer 2

An endonuclease that. creates a blunt end cut at a site specified by guide RNA

Question 3

KEY TERM - CRISPR

Answer 3

Short, clustered repeats of DNA found in prokaryotes which protect the against viral invasion.

Question 3

KEY TERM - Spacer

Answer 3

Short sequences of DNA Obtained from invading Bacteriophages that are added into the CRISPR sequence

Question 4

KEY TERM - Protospacer

Answer 4

A short sequence of DNA extracted from a bacteriophage by Cas1 and which has yet to be incorporated into the CRISPR gene

Question 4

KEY TERM - Guide RNA (gRNA)

Answer 4

RNA which has a specific sequence determined by CRIPSR to guide Cas9 to a specific site

Question 4

KEY TERM - single guide RNA (sgRNA)

Answer 4

guide RNA used by scientists in gene editing.

Question 5

Bacteria can be

Answer 5

infected by a virus called bacteriophages

Question 6

is the point of CRISPR-Cas9?

Answer 6

Bacteria do not have an immune system- they have develpoed CRSPR-Cas9 to limit the effect of Bacteriophages

Question 7

What does CRISPR stand for

Answer 7

Clustered regularly Interspaced Short Palindromic Repeats