3.8Nucleic Acids Flashcards

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1
Q

What are the 2 types

A

RNA

DNA

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2
Q

What biological molecules do nucleic acids contain

A

Carbon hydrogen oxygen nitrogen and phosphorous

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3
Q

What is a nucleic acid

A

Large polymers formed from m at nucleotides linked together

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4
Q

What is a nucleotide made up of

A

A phosphate group that’s negatively charged
A nitrogenous base
A sugar - pentose monosaccharide

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5
Q

How do nucleotides join to make polymer chains

And what does it form

A

By condensation reaction to form polynucleotide
Phosphate group of 5th carbon on the pentose sugar forms a convalent bond with the hydroxyl group at the 3rd carbon of teh pwnstose sugar of the adjacent nucleotide
Bonds formed are phosphodiester bonds
Forming a strong sugar phosphate back bone

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6
Q

What does dna stand for

A

Deoxyribonucleic acid

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7
Q

What is the sugar in dna

A

Deoxyribose

It has 1 fewer oxygen Atom than ribose

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8
Q

What are the 4 different dna nucleotides and what are the 2 catagories they fall into

A

Pyrimidines contain thymine and cytosine they are smaller and contain a single carbon ring structure
Purines contain adenine and guanine they are larger and contain a double ring structure

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9
Q

What is the dna double helix

A

The 2 strands of polynucleotides that make up dna which coil up into a helix

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10
Q

How are the 2 strands held together

A

By hydrogen bonds between the bases

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11
Q

What are the base pairing rules and what’s its name

A

A and T bond only making 2 hydrogen bonds
C and G bond only making 3 hydrogen bonds
It’s called complementary base pairing

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12
Q

What does RNA stand for

A

Ribonucleic acid

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13
Q

What does RNA do

A

Has an essential role in the transfer of genetic info from dna to proteins

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14
Q

Why is rna used instead of dna to transfer info

A

RNA is small enough to leave nucleus and travel to Ribosome

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15
Q

What is the sugar and base in rna

A

Sugar is ribose

Base is Uracil and replaces the thymine base

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16
Q

How do you extract dna

A

Grind sample breaking the cell walls
Mix sample with detergent break down cell memebrane
Add salt - breaks hydrogen bonds
Add protease enzyme - break down proteins
Add layer of alcohol - causing dna to precipatate out of solution