36 - GENE THERAPY Flashcards
what is cell hybridization/fusion
how does the HAT medium work as a natural selection marker
selects for the salvage pathway
what happens when you put a TK deficient cell line and an HPRT deficient cell line together in a HAT medium
how is the measles virus receptor mapped in humans
what is gene therapy
characteristics of different viral vectors
what are the two types of gene therapy
what is in vivo vs ex vivo gene therapy
what is ADA-SCID (adenosine deaminase severe combined immunodeficiency disease)
how is an expression vector for ADA-SCID constructed
how was ADA-SCID partially cured but what were the complication
what are issues with conventional gene therapy
what is a tool used to target specific sequences in the genome
what cells can be used to be reprogrammed into stem cells?
no issue with transplanting back into the patient, since these would be derived from the patient’s own cells and then made into stem cells
