3-3: Genomics Flashcards
Who invented “shotgun sequencing” what is it
Craig Venter. Sequence random bits of DNA, computers fit it together
How did Fredrick Sanger go about DNA sequencing
Based on DNApol building complementary strand using mostly normal dNTPs and rare special dNTPs (lack 3’ OH), these label a different way
Determine sequence based on terminating residue
What is annotation
Identifying the gene, their functions. Done by computers. Identify gene based on homology to known gene
What can genome sequencing tell us
Metabolic capabilities of an organism
Virulence/antibiotic resistance genes present
mutations
discover new genes of medical interest
What is metagenomics
Study of complete genetic content of an environmental sample
Genetic info for organisms not culturable in lab
Composition, composition changes
What is transcriptomics
RNA converted to DNA using reverse transcription, which is then sequenced
Compare expression under different conditions
What is proteomics
Mass spectrometry identifies proteins/protein levels
What proteins present under which conditions
