2-7: Bacterial Cell Inclusions and Endospores Flashcards

1
Q

What is an inclusion

A

Body or aggregate within cell, often related to storage

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2
Q

What is a microcompartment

A

Protein shells that encase specific enzymes/metabolites, cofactors

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3
Q

What is the carbon storage polymer

A

Carbon stored as lipids (polymer) which is formed when there is an excess of carbon/energy
Broken down when C needed

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4
Q

The name of the lipids in carbon storage is

A

poly-B-hydroxyalkanoates

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5
Q

Where is inorganic phosphate stored

A

Polyphosphate granules; broken down to produce nucleic acids, phospholipids

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6
Q

Where is excess sulfur stored

A

Sulfur storage granules

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7
Q

What are gas vesicles. eg?

A

Protein structures that keep water/solutes out, let gas in = floatation/buoyancy
Can bring microbe to favourable environment
e.g. cyanobacteria at surface of water = sunlight

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8
Q

What are carboxysomes

A

They concentrate enzymes involved in carbon fixation. Leads to increased efficiency, reduces side reactions

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9
Q

What is an endospore

A

Dormant cell that survives starvation, harsh evironment

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10
Q

What phylum produces endospores

A

Firmicutes (g+)

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11
Q

Are endospores the only kind of spore?

A

NO, diff bacteria create diff spore structures

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12
Q

What are endospores resistant to

A

Heat, radiation, drying, nutrient depletion, chemicals, etc

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13
Q

What is a vegetative cell

A

A metabolically active, growing/diving cell that become endospores upon nutrient deprivation
Can be reactivated/germinate when environment becomes favourable

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14
Q

How do endospores create a stable/resistant core

A

1) Dehydration of the core.
2) Dipicolinic acid
3) Small acid soluble proteins

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15
Q

What is dehydration of the core

A

Water from 80% to <25%. Increases resistance, inactivates enzymes

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16
Q

How do dipicolinic acids help endospores

A

Important in dehydration process, binds/stabilizes DNA

17
Q

How do small acid soluble proteins help endospores

A

only made during sporulation
Bind DNA - make it compact, protect it from damage (UV, denaturation). Act as carbon/energy source during germination

18
Q

What are the structures of the endospore

A

Core = house DNA/ribosome, becomes vegetative cell
Cortex = peptidoglycan layer
Two membranes = “outer membrane” not like g-; no LPS
Coat = protective protein layer`

19
Q

Endospore formation picture on last slide

A

OK

20
Q

Inclusion that allows for buoyancy?

A

Gas vesicles

21
Q

What concentrates enzymes involved in carbon fixation

A

Carboxysomes