2.3 Nucleic Acids Flashcards
DNA
- deoxyribonucleic acid
- makes up genetic material in cells
- codes for proteins
nucleotide
monomer of DNA of a nucleic acid
amino acid
monomer of a protein
nucleotide components/structure
- phosphate group
- sugar group
- nitrogenous base
DNA nitrogenous bases
- Thymine
- Cytosine
- Guanine
- Adenine
RNA nitrogenous bases
- Uracil
- Guanine
- Cytosine
- Adenine
what is the backbone in a DNA molecule called
Phosphate backbone
how do 2 strands of DNA bond together?
through complementary base pairing
C-G T-A
How many hydrogen bonds do C-G form?
3
How many hydrogen bonds do T-A form?
2
Anti parallel DNA strands
when one strand of DNA goes up, the other goes down
Purines
have 2 carbon rings
Adenine
Guanine
Pyrimidines
have one carbon ring
Thymine
Uracil
Cytosine
DNA structure
2 strands
T-A C-G
Deoxyribose
RNA structure
1 strand
U-A C-G
Ribose
DNA replication step 1
- Helix unwinds
Helix unzips
Hydrogen bonds break
DNA replication step 2
- Strands act as templates
Complimentary free flowing nucleotides
DNA replication step 3
- Joined by DNA polymerase
Hydrogen bonds reform
Sugar-phosphate backbone reforms
Covalent bonds
DNA replication step 4
- Semi-conservative
Enzyme which joins nucleotides of new strand
DNA polymerase
Semi-conservative
has one old strand, one new strand
3 theories how DNA replicates
Conservative
Semi-conservative
Dispersive
Dispersive DNA replication
one protein has all old strands
another protein has all new strands
Conservative DNA replication
all DNA strands reform from old strands
semi-conservative replication experiment
- first experiment = N15
- 2nd experient= N14/15
- 3rd experiement= 1/2 N14/15 1/2 N14
- 4th experiment= 25% N14/15 75% N14
DNA
- Gene codes for a protein
- proteins made from amino acids
- 3 bases (triplet) = 1 amino acid
Transcription protein synthesis
- DNA unwinds and unzips
- mRNA attached to DNA (complementary base pairing)
- used as a template
- RNA moves out of nucleus and goes to a ribosome in the cytoplasm
Translation protein synthesis
- mRNA attaches to a ribosome where it is read by tRNA
- tRNA brings the correct amino acids to fit the triplets creating a protein to join together by peptide bonds
Structure of a DNA nucleotide
- Phosphate
- Deoxyribose sugar
- Nitrogenous base
Structural differences between DNA and RNA
RNA has:
- 1 strand
- Base: Uracil
- Ribose sugar
DNA has:
- 2 strands
- Base: Thymine
- Deoxyribose sugar
polymer of nucleotides
nucleic acid