2.1 ISBT 001 (Major Blood Group System) Flashcards
ISBT means?
International Society of Blood Transfusion
What is the blood group for ISBT 001?
ABO Blood group System
What are the Major Blood group?
ABO and Rh
Immunogen Evoke Immune response
T or F
T
All Immunogens are antigens but not all antigens are immunogens
T or F
T
First Person to do Forward and Reverse typing?
Karl Landsteiner
Most Immunogenic blood group and most important?
ABO
ABO is the only blood group with NATURALLY occuring antibody without prior exposure
T or F
T
Foundation to pre-transfusion testing?
ABO compatibility
What type of antibody that is naturally occuring and more predominant during agglutination / ABO?
IgM
Wrong ABO transfusion is the leading cause of death in Hemolytic transfusion Reaction (HTR)
T or F
T
What chromosome creates H and Se Genes?
Chromosome 19
What chromosome creates ABO Genes?
Chromosome 9
Term for does not produce DETECTABLE antigen?
Amorph
Also known as Silent Gene?
Amorph
What are the Silent Genes?
Jk
O
Lu
(Silent JkOL)
All RBC has Type II chain
T or F
T
what are the GLycosyl transferase?
L-fucosyl transferase
N-acetylgalactosaminyl transferase
D-Galactosyl transferase
Glycosyl transferase for H gene?
L-fucosyl transferase
Glycosyl transferase for B gene?
D-galactosyl transferase
Glycosyl transferase for A gene?
N-acetylgalactosaminyl transferase
Glycosyl transferase for AB gene?
N-acetylgalactosaminyl transferase
D-galactosyl transferase
Glycosyl transferase enzyme catalyzes the TRANSFER of immunodominant sugar to the Type I chain on the RBC
T or F
F
Type II chain
A sugar that occupies the terminal position of the Type II chain (At the end)
This indicates the ABO blood type
Immunodominant Sugar
Greatest Amount of H to Least?
O, A2, B, A2B, A1, A1B
Least amount of H to Greatest?
A1B, A1, A2B, B, A2, O
Surface Ag and Plasma Ab for O?
No surface antigen, Anti A and Anti B
Surface Ag and Plasma Ab for A?
A, Anti B
Surface Ag and Plasma Ab for B
B, Anti-A
Surface Ag and Plasma Ab for AB
AB, No Antibody
universal Donor?
O
Universal Reciever?
AB
Highest Incidence to Lowest for ABO blood group?
O, A, B, AB
Forward and Reverse typing is phenotyping or Genotyping?
Phenotyping
Forward typing uses a commercial known ________ of known specificity
Antisera (Antibody)
The antisera use for Forward typing is Polyclonal
T or F
F
Monoclonal
What is the specimen use for Forward typing?
RCS (Red Cell suspension) / RBC
Forward typing detects antibody
T or F
F
Antigen on RBC surface
Dye for Anti A Reagent?
Blue
Dye for Anti B Reagent?
Yellow
Dye for Anti D Reagent?
Clear
What is the Reaction for both Forward and Reverse typing?
Agglutination
Term for Binding of RBC and Antibody?
Sensitization
Term for binding of sensitized RBC and antibody
Latice formation
Forward typing
Known A: 4+
Known B: 0
A
Forward typing
Known A: 0
Known B: 4+
B
Forward typing
Known A: 0
Known B: 0
O
Forward typing
Known A: 3+
Known B: 3+
AB
Backward typing is also known as Slide typing
T or F
F
Tube typing
Backward typing uses what reagent?
2-5% RBC suspension
Serum contains RBC
T or F
F
Backward typing uses how many tubes?
3
KA, KB, KO
Backward typing detects the presence of?
Antibodies (IgM)
What is the Specimen used for Backward typing?
Serum
Both Forward and Reverse are done at What temp?
Room Temp
What antibody is being detected in Room temp?
IgM
Most common ABO RCS?
O
Backward typing:
Known A cell: O
Known B cell: 4+
A
Backward typing:
Known A cell: 4+
Known B cell: 0
B
Backward typing:
Known A cell: O
Known B cell: O
AB
Backward typing:
Known A cell: 4+
Known B cell: 4+
O
