2.1 ISBT 001 (Major Blood Group System) Flashcards

1
Q

ISBT means?

A

International Society of Blood Transfusion

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2
Q

What is the blood group for ISBT 001?

A

ABO Blood group System

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3
Q

What are the Major Blood group?

A

ABO and Rh

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4
Q

Immunogen Evoke Immune response

T or F

A

T

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5
Q

All Immunogens are antigens but not all antigens are immunogens

T or F

A

T

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6
Q

First Person to do Forward and Reverse typing?

A

Karl Landsteiner

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7
Q

Most Immunogenic blood group and most important?

A

ABO

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8
Q

ABO is the only blood group with NATURALLY occuring antibody without prior exposure

T or F

A

T

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9
Q

Foundation to pre-transfusion testing?

A

ABO compatibility

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10
Q

What type of antibody that is naturally occuring and more predominant during agglutination / ABO?

A

IgM

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11
Q

Wrong ABO transfusion is the leading cause of death in Hemolytic transfusion Reaction (HTR)

T or F

A

T

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12
Q

What chromosome creates H and Se Genes?

A

Chromosome 19

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13
Q

What chromosome creates ABO Genes?

A

Chromosome 9

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14
Q

Term for does not produce DETECTABLE antigen?

A

Amorph

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15
Q

Also known as Silent Gene?

A

Amorph

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16
Q

What are the Silent Genes?

A

Jk
O
Lu

(Silent JkOL)

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17
Q

All RBC has Type II chain

T or F

A

T

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18
Q

what are the GLycosyl transferase?

A

L-fucosyl transferase
N-acetylgalactosaminyl transferase
D-Galactosyl transferase

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19
Q

Glycosyl transferase for H gene?

A

L-fucosyl transferase

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20
Q

Glycosyl transferase for B gene?

A

D-galactosyl transferase

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21
Q

Glycosyl transferase for A gene?

A

N-acetylgalactosaminyl transferase

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22
Q

Glycosyl transferase for AB gene?

A

N-acetylgalactosaminyl transferase
D-galactosyl transferase

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23
Q

Glycosyl transferase enzyme catalyzes the TRANSFER of immunodominant sugar to the Type I chain on the RBC

T or F

A

F

Type II chain

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24
Q

A sugar that occupies the terminal position of the Type II chain (At the end)

This indicates the ABO blood type

A

Immunodominant Sugar

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25
Q

Greatest Amount of H to Least?

A

O, A2, B, A2B, A1, A1B

26
Q

Least amount of H to Greatest?

A

A1B, A1, A2B, B, A2, O

27
Q

Surface Ag and Plasma Ab for O?

A

No surface antigen, Anti A and Anti B

28
Q

Surface Ag and Plasma Ab for A?

A

A, Anti B

29
Q

Surface Ag and Plasma Ab for B

A

B, Anti-A

30
Q

Surface Ag and Plasma Ab for AB

A

AB, No Antibody

31
Q

universal Donor?

A

O

32
Q

Universal Reciever?

A

AB

33
Q

Highest Incidence to Lowest for ABO blood group?

A

O, A, B, AB

34
Q

Forward and Reverse typing is phenotyping or Genotyping?

A

Phenotyping

35
Q

Forward typing uses a commercial known ________ of known specificity

A

Antisera (Antibody)

36
Q

The antisera use for Forward typing is Polyclonal

T or F

A

F

Monoclonal

37
Q

What is the specimen use for Forward typing?

A

RCS (Red Cell suspension) / RBC

38
Q

Forward typing detects antibody

T or F

A

F

Antigen on RBC surface

39
Q

Dye for Anti A Reagent?

A

Blue

40
Q

Dye for Anti B Reagent?

A

Yellow

41
Q

Dye for Anti D Reagent?

A

Clear

42
Q

What is the Reaction for both Forward and Reverse typing?

A

Agglutination

43
Q

Term for Binding of RBC and Antibody?

A

Sensitization

44
Q

Term for binding of sensitized RBC and antibody

A

Latice formation

45
Q

Forward typing

Known A: 4+
Known B: 0

A

A

46
Q

Forward typing

Known A: 0
Known B: 4+

A

B

47
Q

Forward typing

Known A: 0
Known B: 0

A

O

48
Q

Forward typing

Known A: 3+
Known B: 3+

A

AB

49
Q

Backward typing is also known as Slide typing

T or F

A

F

Tube typing

50
Q

Backward typing uses what reagent?

A

2-5% RBC suspension

51
Q

Serum contains RBC

T or F

A

F

52
Q

Backward typing uses how many tubes?

A

3

KA, KB, KO

53
Q

Backward typing detects the presence of?

A

Antibodies (IgM)

54
Q

What is the Specimen used for Backward typing?

A

Serum

55
Q

Both Forward and Reverse are done at What temp?

A

Room Temp

56
Q

What antibody is being detected in Room temp?

A

IgM

57
Q

Most common ABO RCS?

A

O

58
Q

Backward typing:

Known A cell: O
Known B cell: 4+

A

A

59
Q

Backward typing:

Known A cell: 4+
Known B cell: 0

A

B

60
Q

Backward typing:

Known A cell: O
Known B cell: O

A

AB

61
Q

Backward typing:

Known A cell: 4+
Known B cell: 4+

A

O