13.1 Extra ni Sir Flashcards
1
Q
Factor influencing sensitization:
- Ratio of serum to cells
What is the minimum ratio?
A
40:1
(2 drops serum : 1 drop of 5% Volume of Solute per volume of solution - Bascially RCS)
2
Q
Factor influencing sensitization:
- Reaction medium
This allows antibody-coated cells to come closer to one another (Lattice formation)
A
Albumin
3
Q
Factor influencing sensitization:
- Reaction medium
Reduces zeta potential
A
LISS
Low ionic strength solution
4
Q
Factor influencing sensitization:
- Reaction medium
This removes a Water molecule
A
Polyethylene Glycol (PEG)
5
Q
Factor influencing sensitization:
- Reaction medium
Other example?
A
Protamine and Polyvinylpyrrolidone
6
Q
pH and Temp also influence sensitization
T or F
A
T
7
Q
What is the optimal temp for sensitization?
A
37C
8
Q
Centrifugation forces Sensitization
T or F
A
T
9
Q
Enzymes also a factor for sensitization
T or F
A
T
