2. Uses of Rabbits in Research

Question 1

Factors that make NZW suitable for research

Answer 1

Tractable disposition
Moderate size
Large peripheral vessels
Breeds easily in captivity
Short gen time

Question 2

Diff between pAbs and mAbs

Answer 2

Mabs can only bind to one antigenic epitope

Pabs can bind to many different epitopes

Question 3

Most commonly used lab animal for pAb production

Answer 3

Rabbits

Question 4

Immunogen

Answer 4

Any molecule capable of generating an immune response when injected into an animal

Question 5

Adjuvant

Answer 5

Compounds that augment the immune response to an immunogen

Question 6

Which routes of administration for immunogens are contraindicated?

Answer 6

Footpad
IP
IV

Question 7

Most challenging route of innoculation, and why

Answer 7

ID, potential for ulcerations, tedious for large volumes

Question 8

5 mechanisms of action needed for an adjuvant

Answer 8

1. Depot effect
2. Immune modulation
3. Antigen distribution
4. Antigen presentation
5. Cytotoxic lymphocyte effect

Question 9

Gold standard adjuvant

Answer 9

Freund’s complete adjuvant

Question 10

What kind of immune response does FCA cause?

Answer 10

Delayed type hypersensitivity

Question 11

Difference between FCA and FIA

Answer 11

FCA has killed mycobacterial cells; FIA does not.

Question 12

VX2 tumor comes from which virus?

Answer 12

Shope’s papilloma virus

Question 13

Shope’s papilloma virus can progress to what neoplasm?

Answer 13

SCC

Question 14

Brown-Pearce carcinoma comes from which organism originally?

Answer 14

Treponema pallidum

Question 15

Greene melanoma comes from what tissue originally?

Answer 15

Pigmented flank organ of Syrian hamster

Question 16

What model is used to study atherosclerosis in the rabbit?

Answer 16

Cholesterol-fed rabbit

Question 17

WHHL

Answer 17

Watanabe heritable hyperlipidemia

Question 18

Cause of WHHL

Answer 18

Defective LDL receptor causes endogenous hyperlipidemia and hypertriglyceridemia

Question 19

SMHL

Answer 19

Saint Thomas Mixed Hyperlipidemic model

Question 20

Differences between WHHL and SMHL

Answer 20

SMHL rabbits have normal LDL receptor and have only mildly elevated TG

Question 21

Limitations of using rabbits to study ocular disease

Answer 21

Thin cornea
Large lens
Rod-rich, poorly vascularized corneas

Question 22

Pyrogen

Answer 22

Substance causing induction of a febrile response

Question 23

Rabbit Pyrogen Test has been replaced by what test?

Answer 23

Limulus Amoebocyte Lysate Assay