2. Uses of Rabbits in Research Flashcards
Factors that make NZW suitable for research
Tractable disposition Moderate size Large peripheral vessels Breeds easily in captivity Short gen time
Diff between pAbs and mAbs
Mabs can only bind to one antigenic epitope
Pabs can bind to many different epitopes
Most commonly used lab animal for pAb production
Rabbits
Immunogen
Any molecule capable of generating an immune response when injected into an animal
Adjuvant
Compounds that augment the immune response to an immunogen
Which routes of administration for immunogens are contraindicated?
Footpad
IP
IV
Most challenging route of innoculation, and why
ID, potential for ulcerations, tedious for large volumes
5 mechanisms of action needed for an adjuvant
- Depot effect
- Immune modulation
- Antigen distribution
- Antigen presentation
- Cytotoxic lymphocyte effect
Gold standard adjuvant
Freund’s complete adjuvant
What kind of immune response does FCA cause?
Delayed type hypersensitivity
Difference between FCA and FIA
FCA has killed mycobacterial cells; FIA does not.
VX2 tumor comes from which virus?
Shope’s papilloma virus
Shope’s papilloma virus can progress to what neoplasm?
SCC
Brown-Pearce carcinoma comes from which organism originally?
Treponema pallidum
Greene melanoma comes from what tissue originally?
Pigmented flank organ of Syrian hamster
What model is used to study atherosclerosis in the rabbit?
Cholesterol-fed rabbit
WHHL
Watanabe heritable hyperlipidemia
Cause of WHHL
Defective LDL receptor causes endogenous hyperlipidemia and hypertriglyceridemia
SMHL
Saint Thomas Mixed Hyperlipidemic model
Differences between WHHL and SMHL
SMHL rabbits have normal LDL receptor and have only mildly elevated TG
Limitations of using rabbits to study ocular disease
Thin cornea
Large lens
Rod-rich, poorly vascularized corneas
Pyrogen
Substance causing induction of a febrile response
Rabbit Pyrogen Test has been replaced by what test?
Limulus Amoebocyte Lysate Assay
