17. DNA replication Flashcards

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1
Q

The genetic material of which kind of cells is included in a single circular molecule of DNA?

A

Bacteria yoooo

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2
Q

Two sides of double helix structure of DNA are held together with hydrogen bonds

A

True beans

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3
Q

What statement about DNA is false?The two strands are separated.
Each strand is used as a template for making a new duplex.
The synthesis on each strand is in the opposite direction.
The newly synthesized DNA is packaged into one nucleus, and the old DNA is packaged into another nucleus.

A

The newly synthesized DNA is packaged into one nucleus, and the old DNA is packaged into another nucleus.

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4
Q

In rep of DNA, the helix is opened and untwisted by

A

helicase

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5
Q

Since the first nucleotid s cannot be linked In a newly synthesised strand in DNA replication what is required??

A

RNA primer

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6
Q

Enzyme that catalysed new DNA molecules

A

DNA polymerase

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7
Q

True statement about the elongation of the lagging strand during DNA replication

A

Synthesised discontinuouslyokazaki fragments are used to elongate LAGGING strand

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8
Q

What joins DNA FRAGMENTS to the lagging strands??

A

Ligases

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9
Q

DNA is found

A

In greatest quantities in nucleus of cells

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10
Q
  1. Which statement about the elongation of the lagging strand during DNA replication is correct?It is synthesized in a 3’ –> 5’ direction.It requires one RNA primer to proceed.It is synthesized by DNA ligase.It is synthesized discontinuously.
A

It is synthesized discontinuously

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11
Q
  1. Okazaki fragments are used to elongate
A

the lagging strand

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12
Q

When is DNA replicated in cell cycle??

A

Interphase, preparing for cell division

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13
Q

DNA shape in prokaryotic and eukaryotic cells

A

PRO- circular

EUKARYOTIC: linear

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14
Q

What is principle difference in DNA replication between prokaryotic and eukaryotic cells in terms of replication fork???

A

PROKARYOTIC- 1 replication fork ( 1 origin)

ETIC: multiple replication fork ( +1 origin)

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15
Q

What is function of topoisomerase??

A

Alters the supercooled DNASE ahead of replication fork

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16
Q

Describe function of enzymes / proteins in DNA replication (6)

A
  1. HELICASE: unwinds DNA
  2. SSB: stabilises unwond DNA
  3. PRIMASE: make RNA primer
  4. DNA POL III: add new nucleotide
  5. DNA POL II: Break down RNA primer, replace with DNA nucleotide
  6. DNA LIGASE: joins Okazaki fragments
17
Q

What strand is leading in DNA

A

5’ - 3’

18
Q

Lagging strand in DNA

A

3’-5’

19
Q

What is the problem at end of each linear molecules after each replication.. And two solutions to this. (DNA TERMINATION)

A

Linear molecules gets shorter.

Solution to this is telomeres ( NO GENES)

Telomerase (LENGTHENS)