1.3 gene expression Flashcards

1
Q

Gene expression definition

A

where genetic information of a gene of DNA is transcribed and translated to synthesise products such as proteins.

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2
Q

Three types of RNA

A

mRNA
tRNA
rRNA

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3
Q

mRNA definition

A

The type of RNA that is involved in transcribing a gene of DNA and transporting it from the nucleus to the ribosome.

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4
Q

tRNA function

A

tRNA carries an amino acid to the ribosome in translation to bind with mRNA

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5
Q

rRNA function

A

To be part of the ribosome

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6
Q

Triplet definition

A

Three bases of RNA

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7
Q

Codon definition

A

A triplet of bases on mRNA that code for an amino acid when mRNA binds with tRNA

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8
Q

Anti Codon

A

Three bases of tRNA which bond with mRNA.

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9
Q

Differences between RNA and DNA

A

RNA is single stranded whereas DNA is double stranded
RNA contains ribose sugar whereas DNA contains deoxyribose sugar
RNA contains Uracil whereas DNA contains thymine

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10
Q

Transcription stage 2

A

Free RNA nucleotides approach the unzipped DNA strands.

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11
Q

Order of gene expression

A

DNA
Transcription
mRNA
translation
tRNA
Ribosome
Protein

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12
Q

Transcription definition

A

The process of making an RNA copy of a gene of DNA

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13
Q

Transcription stage 1

A

DNA unwinds and RNA polymerase unzips the molecule by breaking apart the weak and temporary hydrogen bonds, exposing the bases.

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14
Q

Transcription stage 3

A

Free RNA nucleotides find the complimentary bases on the DNA strand

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15
Q

Stage 4 of Transcription

A

Weak and temporary hydrogen bonds form between the bases of DNA and the bases of the RNA free nucleotides.

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16
Q

Stage 5 of transcription

A

The enzyme RNA polymerase bonds together the sugar phosphate backbone of the RNA strand

17
Q

Stage 6 of transcription

A

Weak and temporary hydrogen bonds break between the DNA and RNA, and then an mRNA primary transcript strand is formed, DNA redwinds into a double stranded helix.

18
Q

Stage 7 of transcription

A

The mRNA primary transcript undergoes RNA splicing, this is where introns which are non coding regions of RNA are removed from the mRNA strand and the exons, which are coding regions of RNA are compacted together, making the mature transcript strand small enough to leave the nucleus.

19
Q

Stage 1 of translation

A

The mRNA strand leaves the nucleus and travels to the ribosome.

20
Q

Stage 2 of translation

A

The tRNA strand collects an amino acid and travels to the ribosomes to bond with mRNA

21
Q

Stage 3 of translation

A

The codon of mRNA bonds with the anti codon of tRNA using complimentary base pairing.

22
Q

Stage 4 of translation

A

Peptide bonds form between the adjacent amino acids, forming a polypeptide chain.

23
Q

Stage 5 of translation

A

The tRNA strand will then collect another amino acid to repeat the process.

24
Q

Alternative splicing definition

A

Where a different set of exons are spliced together forming a different mature transcript and a different protein in RNA splicing.

25
Q

Polypeptide definition

A

A chain of amino acids held together by peptide bonds

26
Q

What is the protein function determined by

A

The protein shape and structure.

27
Q

What are phenotypes determined by

A

The proteins produced in gene expression

28
Q

How do polypeptide chains become proteins

A

The polypeptide chain folds together and hydrogen bonds bind the individual amino acids in place.