1.1f - Aseptic Technique and Cell Culture Flashcards
Aseptic techniques eliminate…
unwanted microbial contaminants when culturing micro-organisms or cells
Aseptic technique involves…
the sterilisation of equipment and culture media by heat or chemical means and subsequent exclusion of microbial contaminants
A microbial culture can be started using…
an inoculum of microbial cells on an agar medium, or in a broth with suitable nutrients
Many culture media exist that promote the growth of…
specific types of cells and microbes
Animal cells are grown in growth media containing…
growth factors from serum
Growth factors are proteins that…
promote cell growth and proliferation
Growth factors are essential for…
the culture of most animal cells
In culture, primary cell lines can divide…
a limited number of times
In culture, tumour cell lines can divide…
an unlimited number of times
Plating out a liquid microbial culture on solid media allows…
the number of colony-forming units to be counted and the density of cells in the culture estimated
Serial dilution is often needed to…
achieve a suitable colony count
Haemocytometers are used to…
estimate cell numbers in a liquid culture
The haemocytometer chamber has a known volume so…
cell concentration can be calculated from the cell count
Vital staining is required to…
identify and count viable cells
Vital staining only stains…
dead cells
