1.1d & 1.1e - Detecting Proteins Using Antibodies & Microscopy Flashcards
Immunoassay techniques are used to…
detect and identify specific proteins
These techniques use stocks of antibodies with the same…
specificity
Antibodies with the same specificity are called…
monoclonal antibodies
An antibody specific to the protein antigen is linked to…
a chemical ‘label’
Chemical ‘labels’ are often a reporter enzyme, producing…
a colour change
To produce a colour change what can be used?
chemiluminescence, fluorescence and other reporters
In some cases, the assay uses a specific antigen to…
detect the presence of antibodies
Western blotting is…
a technique used after SDS-PAGE electrophoresis
The separate proteins from the gel are transferred (blotted) onto…
a solid medium
The proteins can be identified using…
specific antibodies that have reporter enzymes attached
Bright-field microscopy is commonly used to observe…
whole organisms, parts of organisms, thin sections of dissected tissue or individual cells
Fluorescence microscopy uses specific fluorescent labels to…
bind to and visualise certain molecules or structures within cells or tissues
