(Paramyxoviridae) 1. What order? (structure) 2. What is the symmetry? 3. genome? 4. 15k genome codes for how many proteins? 5. What is the replication site? 6. How do they get out of cell? 7. Does it have spikes? 8. what does nucleic acid look like inside?

1. mononegavirales 2. helical capsid 3. (-) ssRNA 4. 10-12 proteins 5. cytoplasmic replication 6. budding from the plasma membrane 7. yes 8. helical

(Newcastle Disease (Avian Paramyxovirus 1)) 1. Disease of birds including domestic poultry that affects what three areas? 2. Contagious? 3. How stable? 4. Devastating epidemics in poultry 5. one of the major threats to poulty industry 6. need to report?

1. Respiratory, GI, CNS 2. highly (worldwide) 3. stable (persists in flocks and carcasses) 6. maybe

(Pathogenesis of Newcastle Disease (AP1)) 1. Replicates where? (three strains) 2. mild 3. intermediate 4. highly virulent 5. zoonotic?

1. epithelial cells of upper respiratory and intestinal tracts 2. lentogenic 3. mesogenic 4. velogenic 5. mildly (conjuctivitis)

(Pathogenesis of Newcastle Disease (AP1)) 1. incubation period? 2. growth?

1. 4-6 days 2. leads to poor growth - poor egg production

(Diagnosis of Newcastle Disease) 1. Clinical signs - combo of what three things? 2. Gross lesions - hemorrhage where?

1. respiratory, diarrhea, and CNS signs 2. trachea and gizzard

(Canine Distemper (Morbillivirus) 1. Most important disease of dogs? 2. most common when? 3. Mortality and morbidity in unvaccinated? 4. Labile virus - readily destroyed by what?

1. yes 2. 4-6 months of age 3. high in both 4. disinfectants

(Pathogenesis of Canine Distemper cont) 1. virus shed within what? 2. What is the mortality rate? 3. What form is most serious in dogs?

1. all body secretions 2. 30-80% 3. CNS (may have lingering CNS effects)

Rinderpest (Morbillivirus) 1. Is a virus serologically related to what other two viruses? 2. Host range? 3. where at? 4. Labile? what does this mean?

1. canine distemper and measles virus 2. cattle, water buffalo, sheep, goats, camels, pigs 3. mostly Africa 4. yes - can be destroyed by disinfectants

(11) Paramyxoviridae Flashcards by Scott Venhuizen

(Paramyxoviridae)

What order?

(structure)

What is the symmetry?
genome?
15k genome codes for how many proteins?
What is the replication site?
How do they get out of cell?
Does it have spikes?
what does nucleic acid look like inside?

mononegavirales
helical capsid
(-) ssRNA
10-12 proteins
cytoplasmic replication
budding from the plasma membrane
yes
helical

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(Virion Proteins)

1-2. What are the two classes of proteins?

membrane proteins
nucleocapsid proteins

(then look at this a little bit - I don’t really want to know this - it seems like he is focusing on the fact that different types contain different proteins)

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(Virus Replcation_

learn this

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1-2. What are the two major families of Paramyxoviridae?

Paramyxovirinae
Pneumovirinae

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look at these a little

AND THIS

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(Newcastle Disease (Avian Paramyxovirus 1))

Disease of birds including domestic poultry that affects what three areas?
Contagious?
How stable?
Devastating epidemics in poultry
one of the major threats to poulty industry
need to report?

Respiratory, GI, CNS
highly (worldwide)
stable (persists in flocks and carcasses)
maybe

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(Pathogenesis of Newcastle Disease (AP1))

Replicates where?

(three strains)

mild
intermediate
highly virulent
zoonotic?

epithelial cells of upper respiratory and intestinal tracts
lentogenic
mesogenic
velogenic
mildly (conjuctivitis)

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(Pathogenesis of Newcastle Disease (AP1))

incubation period?
growth?

4-6 days
leads to poor growth - poor egg production

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(Diagnosis of Newcastle Disease)

Clinical signs - combo of what three things?
Gross lesions - hemorrhage where?

respiratory, diarrhea, and CNS signs
trachea and gizzard

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(Canine Distemper (Morbillivirus)

Most important disease of dogs?
most common when?
Mortality and morbidity in unvaccinated?
Labile virus - readily destroyed by what?

yes
4-6 months of age
high in both
disinfectants

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(Pathogenesis of Canine Distemper cont)

virus shed within what?
What is the mortality rate?
What form is most serious in dogs?

all body secretions
30-80%
CNS (may have lingering CNS effects)

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Look at this slide

and this one

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(Diagnosis of Distemper)

1-3. What three ways is this done?

paired serology: ELISA, IFA, VN
Localization of viral antigen by IFA
Virus Isolation from buffy coat (difficult)

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(Control of Canine Distemper)

he just sped right through this

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Rinderpest (Morbillivirus)

Is a virus serologically related to what other two viruses?
Host range?
where at?
Labile? what does this mean?

canine distemper and measles virus
cattle, water buffalo, sheep, goats, camels, pigs
mostly Africa
yes - can be destroyed by disinfectants

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Rinderpest (Morbillivirus)

mortality
Signs?
recovery?

90-100%
Fever, weakness, depression, respiratory distress, diarrhea
4-5 weeks (immune of rlife)

Rinderpest (Morbillivirus)

Rinderpest (Morbillivirus)

(Control of Rinderpest)

Importation from endemic countries forbidden
What is used in endiemic countries?

-
attenuated live vaccines

don’t think we really need to know this

(Bovine Respiratory Syncytial Virus (BRSV))

genus what?
Causes disease in cattle of what age?
Major cause of what in calves during the first year of life?
During outbreak… morbidity? mortality?
may infect what else?

genus pneumovirus
cattle of all ages
respiratory diseases
high, usually low
sheep and goat

(Bovine Respiratory Syncytial Virus (BRSV))

(Clincal Disease)

Subclinical infections may occur

ach - just read this

(Bovine Respiratory Syncytial Virus (BRSV))

(Bovine Respiratory Syncytial Virus (BRSV))

(Epidemiology)

common when cattle and sheep confined together
stress of transportation
common when?
spreads through what?
Existing immunity reduces clinical disease

winter
respiratory secretions

(Bovine Respiratory Syncytial Virus (BRSV)) (Diagnosis) - just look at this (Control) 1. What types of vaccines are used in calves?

1. modified live or inactivated

(Avian metapneumocirus (aMPV)) 1. TRT = ? 2. Disease of where? drop in what? what happens to immune system? 3. What is the reservoir host?

1. turkey rhinotracheitis 2. upper respiratory disease, drop in egg production, gets suppressed 3. canadian geese (note the spread)

(Avian metapneumocirus (aMPV)) (Pathogenesis) 1. Virus replicates where? 2. virus detectable during what phase? how long? 3. What are the clinical signs? (Control) 4. What vaccine is used?

1. upper respiratory tract 2. acute phase (1 week) 3. respiratory distress and immunosuppression 4. modified live virus

(Blue eye disease of swine in MExico) 1. Caused by what virus? 2. enveloped or no? DNA or RNA? hemagglutination with bovine RBC? 3. Syncytia formation in what cells? 4. six structural proteins 5. young pigs - encephalitis, pneumonia, and corneal opacity 6. pregnant sows and boars - reproductive failures

1. porcine rubulavirus 2. enveloped RNA; yes 3. PK-15 cells

(Henipavirus) 1. genus of the family what? 2. What are the two established species? 3. What is the natural host? 4. Are these **zoonotic**? 5. are these a reportable disease? 5. originally reported in australia and asia

1. paramyxoviridae 2. hendra virus and nipah virus 3. pteropid fruit bats 4. yes - cause disease and death in both animals and humans 5. yes

just read these

and this