(10) Rhabdoviridae Flashcards
(Rhabdoviridae)
- What shape are they?
- RNA or DNA?
- how many species?
- bullet-shaped
- RNA
- 175
(Rhabdoviridae)
1-3. What are the three genera that he wants us to know
- lyssavirus (rabies virus)
- vesiculovirus (vesicular stomatitis virus)
- novirhabdovirus (viral hemorrhagic septicemia virus)
(Rhabdoviridae structure)
- what does the genetic material look like?
- Are they enveloped or non? feature what?
- G protein is a target of what?
- what shape is the nucleocapsid?
- encodes 5 genes
- non-segmented negative sense ssRNA
- enveloped; feature large glycoprotein spikes
- neutralizing antibodies
- helical cylindrical nucleocapsid bullet
(Genus: Lyssavirus - continued)
- capable of causing what diseas?
- cross-reacting antigenic sites and distinct virus neutralization epitopes
- What are common hosts for these viruses?
- rabies like disease
- bats (for all lyssa viruses - except for Mokola I guess)
What are neutralizing antibodies?
they stop the virus from entering the cell
(for example PRRSV you get neutralizing antibodies that can’t stop infection - with rabies the neutralizing antibodies do stop infection)
(Rabies Virus)
- what can be infected?
- fatal?
- What are the most important source of human rabies worldwide?
- all warm-blooded animals
- fatal
- dogs
(Rabies Pathogenesis)
- transmitted by what?
- alot of infectious virus where?
- once virus enters the brain is it 100% fatal?
- aersol transmission possible? organ transplant?
- How long is the incubation period?
- transmitted by bite or scratch
- saliva
- yes
- yep, yep
- 14 to 90 days (up to two years in some cases) - needs to move from peripheral site to CNS
(Rabies Pathogenesis)
1-4. The probability that an animal develops overt rabies post exposure depends on what factors?
- dose
- variant of virus
- species of animal
- location and severity of bite
(Rabies Pathogenesis: Virus Entry)
- Can rabies infect neuronal and non-neuronal cells?
- Nonspecific attachment via what cell receptors (2 of them)?
- Specific binding via what 3 receptors?
- yes
- phopholipids and gangliosdies
- nicotinic acetylcholine receptor (primary), neural cell adhesion molecule, P75 neurotropin receptor
- Rabies starts with what?
- Travels to where via what?
- destroys what? causing what?
- travels out via nerves to what? to do what?
- What site is the most critical?
- can saliva be infectionus before symptoms?
- replication at site of infection
- brain via retrograde transport through axons
- limbic system - emotional behavior
- salivary glands (and adrenal cortex and pancreas); so it can affect more things
- the salivary glands (at the apical surface)
- yes
look at this - afraid of water - fucking crazy duuuuudddeee
(rabies - clinical disease)
- What are the two clinical forms of rabies?
- Which animals exhibit which types more?
- What is the phase before clinical disease called?
- furious form (targets limbic system) and dumb (paralytic form) (targets neocortex)
- dogs, cats, and horses are furious - ruminants and lab animals are dumb (but both forms can be seen in both)
- prodromal phase (usually overlooked)
i don’t know the answer to this
negri bodies are an accumulation of rabies virus nucleocapsids in nerouns - seen as cytoplasmic inclusions
this will be on test!!
rabies
take a gander
- Can you diagnose rabies with just negri bodies?
- yes (but you have to be good at it)
Immunofluorescnce assay is used - must send fresh samples
is there zoonotic potential?
YES!!!!
read this
and this
and this
and then read about VSV - but don’t memorize every fucking word of it
