(06) Viral Diagnosis

Question 1

(Diagnosis of Viral Diseases)

1-2. What are the two major approaches for Viral Diagnosis?

Answer 1

1. locate the infectious virus, viral antigen, or viral nucleic acid
2. Demonstrate the presence of virus-specific antibodies

Question 2

just read this slide

Question 3

Collection of specimen

1. What are five imporant things in collection?

Answer 3

1. timing, clinical signs, knowledge of pathogenesis, transport, sample processing

Question 4

take a look at this table - kind of know it i guess - this class confuses me

Question 5

1. What is the first way to diagnose a virus?
2. In viruses there are certain viruses that cause what? What is it? where? how do you see them?

3, What is a negri body?

Answer 5

1. by gross evaluation and histopathology
2. inclusion bodies; a collection of virions; within cells; histologically
3. an inclusion body in rabies (there is a picture of this as well)

this is herpes

take a look at the other histo slides

Question 6

(Methods of Detection of Viruses)

get a good idea of these - don’t flat out memorize them!

1. In today’s world what is the most common test used for detection? what is the problem with this tool?

Answer 6

1. PCR (detection in nucleic acid) - have to know what you are looking for

Question 7

1. an eosinophilic virus inclusion body is what?

Answer 7

1. intracellular collection of virions

Question 8

(Direct Identifcation of Virus or Viral Genome)

1. name three of these

Answer 8

1. EM, isolation of live virus, polymerase chain reaction (PCR)

Question 9

(EM)

1. requires highly trained personell and equipment. high cost. low sensitivy. visual image
2. value?

Answer 9

2. low value

Question 10

(Isolation of Live Virus)

1. commonly used method for diagnosing what?
2. Virus must be able to do what?

Answer 10

1. viral infections
2. grow in cells

Question 11

(PCR) polymerase chain reaction

1. HIgh tech, specific
2. used routinely?
3. need to know what you are looking for?

Answer 11

1. -
2. yes
3. yes