1 Genetic Engineering

Question 1

develops new varieties of organisms by using the process of selection

Answer 1

CLASSICAL BREEDING

Question 2

seeks to achieve expression of genetic material which is already present within a species.

Answer 2

CLASSICAL BREEDING

Question 3

works primarily through insertion of genetic material.

Answer 3

GENETIC ENGINEERING

Question 4

Employs processes that occur in nature, such as sexual and asexual reproduction.

Answer 4

CLASSICAL BREEDING

Question 5

The insertion process in genetic engineering does not occur in nature.

Answer 5

GENETIC ENGINEERING

Question 6

Emphasizes desirable characteristics/ gene.These characteristics are not new for the species.

Answer 6

CLASSICAL BREEDING

Question 7

Involves a direct change to an organism’s genome.

Answer 7

GENETIC ENGINEERING

Question 8

Genetic Engineering deals with the modification of genetic material of an organism. Also known as ______________________________

Answer 8

Recombinant DNA Technology

Question 9

Is a technology that is used to insert a human gene into the genetic material of a common bacteria.

Answer 9

Recombinant DNA Technology

Question 10

Steps of Molecular Cloning/ Recombinant DNA

Answer 10

1. Identifying which chromosome contain the gene of interest.
2. Selection of appropriate vector or vehicle which would propagate the recombinant DNA.
3. Cutting a cleavage DNA by Restriction enzymes.
4. Ligation of the gene of interest with the vector.
5. Transfer of recombinant plasmid into the host.
6. Selection process to screen which cell contain the gene of interest.

Question 11

QUALIFICATION OF A GOOD VECTOR

Answer 11

1. Small and easy to isolate.
2. Can be introduced into a cell.
3. Not toxic to host cell
4. Have one or more origins of replication.
5. Have one or more unique restriction sites.
6. Have a selectable marker (antibiotic resistance gene) which allows recognition of transformants.

Question 12

Methods to introduce DNA into cells

Answer 12

1. Transformation
2. Transfection
3. Biolistics/ gene gun

Question 13

STEPS OF TRANSFORMATION - PROKARYOTIC CELLS

Answer 13

1. Mix the Plasmid and E. Coli
2. Soak in cold temperature (30 mins)
3. Soak in high temperature (42 Celcius)

And it will create temporary pores

Question 14

Methods of Transfection

Answer 14

1. Chemical Transfection
2. electroporation

Question 15

TRANSFECTION - _________CELLS

Answer 15

EUKARYOTIC

Question 16

Note that in letting the recombinant bacterium culture 2 things may happen

Answer 16

1. Some may not contain the recombinant DNA
2. Some may produce a non- recombinant DNA