04 - Parasitology Flashcards
Term: presence of an endoparasite in host
infection
Term: presence of an ectoparasite in host
infestation
Type of parasite that needs a host at some stage of life cycle to complete development and propagation.
Obligate parasite
Type of parasite that may exist in a free-living state but becomes parasitic when the need arises
Facultative parasite
This is a type of parasite that can establish itself in a host where it does not ordinarily live in.
Accidental or Incidental parasite
Type of parasite which successfully invades a host but merely passes through the GI tract without colonizing it.
Spurious parasite
Type of host in which the parasite attains sexual maturity.
Definitive (or Final) host
Type of host which only harbors the asexual or larval stage.
Intermediate host
Type of host in which the parasite does not develop further into later stage.
Paratenic host
Type of host in which the life cycle of the parasite is allowed to continue and which become additional sources of human infection
Reservoir host
What are vectors?
2 types of vectors, and describe.
Vectors transmit parasites from one host to another.
- Biologic vector - transmits a parasite only after it has completed its development (thus, also a host)
- Mechanical (or Phoretic) vector - only transports the parasite
Term: infection wherein an infected individual is further infected with the same species, leading to massive infection
Hyperinfection, a.k.a. Superinfection
What is the cause/origin of congenital toxoplasmosis?
Exposure of mother to cats during pregnancy
enumerate: virulence factors of Entamoeba histolytica, and their functions
- lectin - mediates adherence
- amebapores - for penetration
- cysteine proteases - most important virulence factor, which produces ulceration with bleeding
flask-shaped colon ulcers
Amebic colitis
anchovy sauce-like aspirate
Amebic liver abscess
Identify: most invasive among the Entamoeba genus.
Entamoeba histolytica
These amebicides act on organisms in the bowel wall and the liver. Give examples
- Tissue amebicides
- chloroquine, emetines, metronidazole, tinidazole
These amebicides act only in the lumen of the bowel. Give examples
-Luminal amebicides -diloxanide furoate, iodoquinol, paromomycin
Drug of choice for asymptomatic luminal cysts of E. histolytica:
Diloxanide furoate
Drug of choice for severe intestinal and extraintestinal amebiasis:
Metronidazole
Pathologic effect of Giardia infection:
acute effects
chronic effects
- attach and causes pathologic changes to the intestinal villi (villous flattening, crypt hypertrophy, and disruption of cytoskeleton), causing malabsorption
- Acute infection: flatulence, abdominal pain, diarrhea
- Chronic infection: steatorrhea, constipation, weight loss
Treatment of Giardiasis:
Metronidazole
How is Giardia lamblia transmitted?
Ingestion of food/water from sources contaminated with feces containing cysts
Gold standard for diagnosis of Giardia lamblia
direct fluorescent antibody testing
This parasite cause Gay bowel syndrome. Why is this so?
Giardia lamblia - high prevalence in homosexuals due to to their oro-anal practices
- Manifestation of cryptosporidiosis
- Cryptosporidiosis is life-threatening in what condition: and why?
- self-limited non-bloody diarrhea
- CD4 < 200, due to autoinfection
Treatment of cryptosporidiosis:
Usually self-limiting; DOC is Nitozoxanide, but in HIV patients, treatment with antiretrovirals may help the patient fight the pathogen on his own
Identidy: characterized by foul-smelling, greenish vaginal discharge, accompanied by itching and burning; with a characteristic strawberry cervix; What is the pathogen?
Trichomoniasis - cause by Trichomonas vaginalis
The only protozoal form of Trichomonas vaginalis
Trophozoite
How is trichomoniasis transmitted?
sexual intercourse (ping-pong transmission)
Treatment for trichomoniasis
Single dose 2 g oral metronidazole
most important parasitic disease in man
Plasmodium
What is the vector for Plasmodium?
Anopheles flavirostris minimus (infected female)
Two processes in the asexual life cycle of Plasmodium
- shizogony - occurs inside the RBCs
- gametogony
Process in the sexual life cycle of Plasmodium. Where does it occur?
sporogony - occurs inside the female mosquito (Anopheles flavirostris minimus)
What conditions are said to be protective against Plasmodium infection? (immune to malaria)
defects in RBC morphology (G6PD deficiency, sickle cell disease)
Term: recurrence of symptoms after a temporary abatement. Which Plasmodium species exhibit this?
- Recrudescence
- P. falciparum and P. malariae
- Term: return of a disease after its apparent cessation.
- Which Plasmodium species exhibit this?
- In Plasmodium, how does this happen?
- Relapse
- P. ovale and P. vivax
- due to reactivation of hypnozoites
Identify: Plasmodium: intermediate host / definitive host
humans / Anopheles flavirostris minimus
Gold standard for diagnosis of Plasmodium:
Giemsa stain:
- Thick smear - to screen for presence of organisms
- Thin smear - to identify the species of the infecting organism
- Identify: Punctate granulations present in red blood cells that contain hypnozoites.
- What Plasmodium species causes these changes?
- Schuffner dots
- Plasmodium ovale and Plasmodium vivax
- Identify: coarse granulations present in red blood cells.
- What Plasmodium species causes these changes?
- Maurer dots
- Plasmodium falciparum
- Identify: fine dots present in red blood cells.
- What Plasmodium species causes these changes?
- Ziemann dots
- Plasmodium malariae
Ziemann is a Fine Man
A severe complication of P. falciparum malaria, characterized by intravascular hemolysis, massive hemoglobinuria, and acute renal failure
blackwater fever
A severe form of malaria that is accompanied by septic shock.
Algid malaria
Enumerate: Usual symptoms of malaria (5)
- malaise
- joint pains
- hemolytic anemia
- jaundice
- splenomegaly
Term: These antimalarial drugs kill schizonts in the liver. Give some examples
- Tissue Schizonticides
- primaquine
Term: These antimalarial drugs kill the parasitic forms only in the RBCs. Give some examples
- Blood schizonticides
- chloroquine, quinine
Term: These antimalarial drugs kill gametocytes in human blood. Give some examples
- gametocides
- primaquine
Term: These antimalarial drugs prevent sporogony and multiplication in the mosquito. Give some examples
- sporonticides
- proguanil, pyrimethamine
Per Plasmodium species, in terms of erythrocyte age, what erythrocytes do they target? (young vs old)
- vivax - young RBCs
- ovale - young RBCs
- malariae - old RBCs
- falciparum - all ages
Vata, oVata, Matanda, Futa
Form of heme that is non-toxic to the Plasmodium cell
Hemozoin
- Definitive host of Toxoplasma gondii
- DOC
- domestic cat
- sulfadiazine and pyrimethamine
How is Toxoplasma transmitted?
- ingestion of food/water with fecal oocysts
- transplacentally
Two types of trophozoites of Toxoplasma
- Tachyzoites - rapidly multiplying
- Bradyzoites - slowly multiplying
Enumerate: signs of Congenital toxoplasmosis
- hydrocephalus
- encephalitis
- chorioretinitis
- hepatosplenomegaly
vector of Trypanosoma cruzi
reduviid (Triatoma) bug bite
Tissue that is the most frequently and severely affected by T. cruzi
cardiac tissue
What is the Romana sign?
- periorbital edema in acute Chagas disease
What is a chagoma?
inflammatory nodule near the bite, in acute Chagas disease
Enumerate: manifestations of chronic Chagas disease:
- myocarditis
- megacolon
- megasesophagus (achalasia)
Treatment for Chagas disease
Nifurtimox
Causative agent of West African sleeping sickness
Trypanosoma brucei gambiense
Causative agent of East African sleeping sickness
Trypanosoma brucei rhodesiense
Which is more rapid and fatal, gambian or rhodesian sleeping sickness?
Trypanosoma brucei rhodesiense
Rapid Rhodesian! Fatallll
- spectrum of disease caused by Trypanosoma brucei:
- indurated skin ulcer (trypanosomal chancre)
- enlargement of posterior cervical LN (winterbottom sign)
- hyperesthesia (Kerandel sign)
- encephalitis - Mott cells
- somnolence
DOC for advanced West African sleeping sickness
Eflornithine
WE - PESM
West / East
Pentamidine, Eflornithine / Suramin-Melarsoprol
It SUR is nice to sleep because of MELAtonin -> Suramin-Melarsoprol
DOC for advanced East African sleeping sickness
Melarsoprol
WE - PESM
West / East
Pentamidine, Eflornithine / Suramin-Melarsoprol
It SUR is nice to sleep because of MELAtonin -> Suramin-Melarsoprol
DOC for early West African sleeping sickness
Pentamidine
WE - PESM
West / East
Pentamidine, Eflornithine / Suramin-Melarsoprol
It SUR is nice to sleep because of MELAtonin -> Suramin-Melarsoprol
DOC for early East African sleeping sickness
Suramin
WE - PESM
West / East
Pentamidine, Eflornithine / Suramin-Melarsoprol
It SUR is nice to sleep because of MELAtonin -> Suramin-Melarsoprol
How is African sleeping sickness transmitted?
bite of the tsetse fly (Glossina)
Term: T. brucei is able to evade the immune response, causing cyclic fever spikes (every how long?) due to the ability to change its surface coat.
Antigenic variation
-every 2 weeks
Causative agent of: cutaneous Leishmaniasis
Leishmania tropica
CuTaneous - TropiCa
Causative agent of: visceral Leishmaniasis
Leishmania donovani
donoVani - Visceral
Another name for visceral Leishmaniasis
Kala-azar
Causative agent of: mucocutaneous Leishmaniasis
Leishmania braziliensis
vector of Leishmania
sandfly (Phlebotomus)
Treatment for Leishmaniasis:
Stibulum gluconate
Enumerate: Free-living ameba
- Acanthamoeba castellani
- Naegleria fowleri
How is N. fowleri transmitted?
by swimming in contaminated pools/ponds
- Identify: the only ciliated protozoans to cause human disease.
- What disease do they cause?
- What is the morphology of the ulcer?
- Balantidium coli
- Balantidial dysentery
- round-based, wide-necked intestinal ulcers
How is Babesia microti transmitted?
bite of the Ixodes tick, typically while hunting or wandering in the woods
- morphology of Babesiosis
- Maltese cross - trophozoites in tetrads
Identify: the largest protozoan parasite
Balantidium coli
Gold standard for diagnosis of Babesiosis?
PCR
Enumerate: Two differential diagnoses for cryptosporidiosis
- Cyclospora cayatanensis
- Isospora belli
- they also cause diarrhea in immunocompromised patients
most common and largest intestinal nematode
Ascaris lumbricoides
Enumerate: soil-transmitted helminths
- Ascaris,
- Hookworms (Ancylostoma and Necator), and
- Trichuris
- Enterobius vermicularis
Enumerate: Parasites with transpulmonary stages
- Ascaris lumbricoides
- Ancylostoma duodenale (hookworm)
- Necator americanus (hookworm)
- Strongyloides stercoralis
enumerate: diagnostic tests for Ascaris lumbricoides (2)
- direct fecal smear
- Kato-katz technique
hypersensitivity pneumonitis (eosinophilia) caused by Ascaris lumbricoides in the lungs
Loeffler’s syndrome
Enumerate: hookworms
- Necator americanus
- Ancylostoma duodenale
Buccal organ of Necator americanus
cutting plate
necator = catting plate
Buccal organ of Ancylostoma duodenale
teeth
Identify: “new world hookworm”
Necator americanus
Identify: “old world hookworm”
Ancylostoma duodenale
Diagnostic tests for hookworms:
Direct fecal smear
Katokatz technique
How much blood is being lost everyday to hookworms?
0.25 mL per worm per day
This substance produced by the hookworms aids in blood-sucking:
Anticoagulant
How are hookworms transmitted?
Hookworms penetrate throught the skin
Serpiginous tracts of hookworms are called:
cutaneous larva migrans
Common term for Trichuris trichiura:
whipworm
How to diagnose Trichuris?
Direct fecal smear
Katokatz technique
Morphology of Trichuris trichiura
barrel-shaped eggs with bipolar plugs
- Spectrum of disease in Trichuris infection
- diarrhea
- rectal prolapse - from increased peristalsis to expel the worms
Most common soil-transmitted helminth in developing countries:
Enterobius vermicularis
Identify: Enterobius vermicularis: intermediate host / definitive host
Its life cycle is confined to HUMANS ONLY
How to diagnose Enterobius vermicularis? What will you see?
Graham scotch tape techinique - D-shaped cells
Describe pathogenesis of Enterobius vermicularis:
Female worms release thousands of fertilized eggs on perianal skin, causing perianal pruritus -> autoinfection
- spectrium of disease for enterobiasis:
- pruritus ani
- eosinophilic enterocolitis
- vulvovaginits
- appendicitis (Oxyuriasis)
Treatment for Enterobius vermicularis
Pyrantel palmoate
How is Strongyloides stercoralis transmitted?
The larvae penetrate the skin.
How to diagnose Strongyloides?
Harada-Mori culture
Infection of Strongyloides on immunicompromised patients results to
Hyperinfection
- Spectrum of disease in acute Strongyloides infection:
- ground itch
- diarrhea
- eosinophilic pneumonia
Term for serpiginous tract of Strongyloides stercoralis:
larva currens
Spectrum of disease in chronic Strongyloides infection:
- serpiginous tracts
- duodenitis
- paradoxical asthma
- hyperinfection syndrome
The only nematode whose life cycle involves a migratory bird:
Capillaria philippinensis
How is Capillaria philippinensis transmitted?
Eating undercooked fish
- How to diagnose Capillaria philippinensis?
- morphology:
- Direct fecal smear
- Kato-katz technique
- peanut-shaped eggs with flattened bipolar plugs
Effects of acute infection of Capillaria?
- borboborygmus
- fever, abdominal pain, eosinophilia
Effects of chronic infection of Capillaria?
- severe malabsorption
- protein-losing enteropathy
- hypogammaglobulinemia
- Identify: this nematodes is able to cause the human host to have a “gurgling stomach”.
- What is the term for this symptom?
- Capillaria philippinensis
- Borborygmus
Most debilitating nematode infection:
Filiariasis
Enumerate: two species of filarial worms
- Wuchererria bancrofti
- Brugia malayi
Usual scenario of filarial infection:
-Farmer from abaca plantation
- Which filarial worm is smoothly curved?
- kinky?
- Wuchereria
- Brugia
Which filarial worm is prevalent only in South East Asia?
Brugia
Mosquito vector of Wuchereria:
- Aedes
- Anopheles
- Culex
Mosquito vector of Brugia
Mansonia
Disease caused by Wuchereria:
Hydrocoele
Disease caused by Brugia:
Elephantiasis
Mechanism of transmission of filarial worms:
mosquito bite
A. Diagnosis of filarial worms:
B. When should blood samples be collected? Why?
A.
- Thick blood smear
- DEC provocation test, allows collection of blood specimens even at daytime
B. Between 8 PM and 4 AM - due to their nocturnal periodicity
Explain the pathogenesis caused by the filarial worms.
Adult worms cause inflammation in the lymph nodes and cause obstruction of the lymphatic vessels, causing lymphedema.
Morphology of granulomas seen in Filarial infection:
Meyers-Kouwenaar bodies
Manifestations of chronic filarial infection:
- hydrocoele
- elephantiasis
- chyluria
What parts of the Philippines experience Bancroftian filariasis?
Sorsogon, Samar, Leyte, Palawan
What parts of the Philippines experience Malayan filariasis?
Eastern Samar
DOC for filariasis:
diethycarbamazine
Identify: Trichinella spiralis: intermediate host / definitive host
pigs / humans
Mechanism of transmission of Trichinella:
consumption of undercooked pork
How to diagnose Trichinosis:
- muscle biopsy (Nurse cells)
- elevated creatine phosphokinase
- xenodiagnosis
Spectrum of disease in trichinosis:
- periorbital edema
- hemorrhagic phenomena (subconjunctival, splinter)
- respiratory myositis
DOC for trichinella
thiabendazole
Leading cause of blindness in sub-Saharan Africa (what organism?)
Onchocerca volvulus
Vector of Onchocerca volvulus:
female blackfly (Simulium)
Clinical finding in Onchocerciasis:
river blindess
What causes the Mazotti reaction?
inflammatory reaction to the lysis of the worms
- Enumerate the 3 trematodes
- Sites of affectation
- intermediate hosts
- Schistosoma japonicum / Paragonimus westermani / Chlonorchis sinensis
- Liver / lung / liver
- snail / snail and crab / snail and fish
How is paragonimiasis transmitted?
Consumption of raw crabs
Treatment of choice for trematodes:
Praziquantel
Common name for Schistosoma japonicum:
oriental blood fluke
Intermediate host of S. japonicum:
Oncomelania snails
How is schistosomiasis transmitted?
the cercariae penetrate the skin
Diagnostic tests for Schistosomiasis (and morphology)
- Katokatz techiniqe
- Circumoval precipitin test (ovoid egg with small hook - lateral knob)
Spectrum of disease in Schistosoma japonicum infection:
- swimmer’s itch
- Katayama fever
- chronic liver disease
Areas of endimicity of S. japonicum:
Leyte, Samar, Sorsogon
Another name for Paragonimus westermani:
lung fluke
Intermediate hosts of Paragonimus westermani:
- snail (Antemelania asperata)
- mountain crab (Sundathelphusa philippina)
Diagnostic test for Paragonimus:
3% NaOH preparation
- Spectrum of disease in Paragonimiasis
- chest X-ray:
- chronic cough with bloody sputum (TB-like)
- ring-shadowed opacity
Two species of Asian Liver flukes:
- Clonorchis sinensis
- Opistorchis viverrini
Intermediate hosts of Clonorchis sinensis:
- snail (Parafossarulus)
- fish (Cyprinidae)
How is Clonorchis sinensis transmitted?
consumption of undercooked/raw fish
Diagnostic tests for Clonorchis sinensis:
morphology:
Direct fecal smear
Potassium permanganate stain (ovoid with melon-like ridges and abopercular protruberance)
Serious complication of chronic Clonorchis sinensis infection:
cholangiocarcinoma
diagnostic stage for Taenia:
gravid proglottid
- Cestode that can cause neurocysticercosis:
- Appearance on CT scan:
- Taenia solium
- ring-enhancing lesion
Manifestations of T. saginatta infestation:
pruritus ani and intestinal obstruction
DOC for Taenia solium
Praziquantel
DOC for Taenia saginata
Praziquantel
- also known as Fish tapeworm
- morphology:
- Diphyllobothrium latum
- sucking grooves and operculated eggs
how is Diphyllobothrium latum transmitted?
Consumption of undercooked fish
Hematologic effect of Diphyllobothrium latum infection:
megaloblastic anemia due to vitamin B12 deficiency (absorbed by the tapeworm)
DOC for Diphyllobothrium latum:
praziquantel
Identify: Echinococcus granulosus: intermediate host / definitive host
sheep or man / dogs
Diagnostic test for Diphyllobothrium latum:
- direct fecal smear
- Katokatz
What is the pathologic effect of hydatid cyst rupture:
severe anaphylaxis
- DOC for Echinococcus granulosis
- Treatment of choice in hydatid cysts
- Albendazole
- Surgical resection