02 Trimming

Question 1

the removal of excess wax from the paraffin block to expose the tissue surface in preparation for actual cutting

Answer 1

trimming

Question 2

trimming is sometimes referred to as “___” or “___ ____”

Answer 2

facing
rough cutting

Question 3

coarse facing is done on the microtome at approximately ___ microns at a time until the entire tissue surface is exposed

Answer 3

15-30

Question 4

during coarse facing, depending upon the size and orientation of the tissue sample, the block surface may measure between ___ micrometers

Answer 4

4-60

Question 5

aggressive coarse trimming will cause “___ ___” artifacts

Answer 5

moth hole

Question 6

fine trimming may be done by either setting the thickness adjust at ___ mm or by advancing the block using the coarse feed mechanism

Answer 6

15 mm

Question 7

during fine trimming, the knife is usually tilted at ___ angulation on a microtome to allow a clearance angle between the cutting facet and the tissue block

Answer 7

0-15 degree

Question 8

___ knives require smaller clearance angles than wedge-shaped knives

Answer 8

biconcave

Question 9

in trimming for ultramicrotomy, the capsule is trimmed while viewing under the ___ ___ using old ___ ___ or knives not suitable for sectioning

Answer 9

dissecting microscope
glass knives

Question 10

in trimming for ultramicrotomy, the angle of the pyramid sides should be about ___

Answer 10

45 degrees

Question 11

the most desirable block face does not exceed __ mm

Answer 11

0.5 mm