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MT6321 > 02 Embedding and Impregnation > Flashcards

02 Embedding and Impregnation Flashcards

1
Q

___C temperature prevents cracking of the tissue block

A

10C

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1
Q

a process of saturating the tissue with a medium, usually liquid paraffin, to permeate or fill up the natural cavities, spaces, and interstices of the tissue

A

impregnation

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2
Q

in embedding, the paraffin should appear ___ and ___ and no layering should be formed

A

clear
homogenous

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3
Q

paraffin rapidly converts from solid to liquid at ___C

A

56-58C

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4
Q

type of paraffin wax:

amorphous, slightly yellowish substance

A

celloidin

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5
Q

the less purified form of celloidin

A

collodion or nitro-cellulose

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6
Q

T/F: celloidin does not require heat

A

T

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7
Q

describe the consistency of celloidin

A

rubbery

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8
Q

substitute for paraffin wax:

mixture of highly purified paraffin and synthetic plastic polymers

A

paraplast

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9
Q

substitute for paraffin wax:

less brittle and less compressible than paraplast

A

embeddol

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10
Q

substitute for paraffin wax:

a semisynthetic wax recommended for embedding eyes

A

bioloid

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11
Q

substitute for paraffin wax:

has a lower melting point (46-48C), but is harder than paraffin

A

ester wax

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12
Q

substitute for paraffin wax:

not soluble in water, but is soluble in 95% ethyl alcohol and other clearing agents

A

ester wax

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13
Q

melting point of water soluble waxes

A

38-42C or 45-46C

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14
Q

substitute for paraffin wax:

a polyethylene glycol is suitable for many enzyme histochemical studies

A

carbowax

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15
Q

in using dimethyl sulphoxide, histotechnologists may experience an unpleasant and annoying ___ or ___ taste

A

oyster
garlic

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16
Q

T/F: sectioning of paraffin is difficult at low temperatures

A

F (high)

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17
Q

type of paraffin wax:

allows higher concentration to be used; greater speed of impregnation

A

low viscosity nitrocellulose (LVN)

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18
Q

type of paraffin wax:

final block is harder, allowing thinner sections to be cut

A

low viscosity nitrocellulose (LVN)

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19
Q

type of paraffin wax:

have a tendency to crack down during handling and staining; highly explosive

A

low viscosity nitrocellulose (LVN)

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20
Q

in double embedding, the tissue is first impregnated with ___, and subsequently blocked in ___

A

celloidin
paraffin wax

21
Q

this provides extra degree of resilience when cutting hard tissues

A

double embedding

22
Q

type of paraffin wax:

main use is in double embedding technique with ester wax or paraffin wax

A

agar

23
Q

type of paraffin wax:

cohesive agent for multiple fragments or friable tissue

A

agar

24
Q

type of paraffin wax:

has a lower melting point than aga

A

gelatin

25
Q

type of paraffin wax:

main use in the production of whole organ sections

A

gelatin

26
Q

type of paraffin wax:

tissues can be embedded directly from water

A

water-soluble waxes

27
Q

type of paraffin wax:

restricted due to the violent diffusion currents which can lead to the complete fragmentation of the section

A

water-soluble waxes

28
Q

plastic embedding medium:

reduces antigenicity, toxic, and damages tissue

A

epoxy

29
Q

epoxy:

bisphenol A

A

araldite

30
Q

epoxy:

glycerol

A

epon

31
Q

epoxy:

cyclohexene dioxide

A

spurr

32
Q

plastic embedding medium:

not often used

A

polyester

33
Q

plastic embedding medium:

used extensively for light microscopy

A

acrylic

34
Q

mold for embedding:

molds for routine work and are widely used

A

leuckhart’s embedding mold

35
Q

mold for embedding:

consists of 2 L-shaped pieces of metal

A

leuckhart’s embedding mold

36
Q

mold for embedding:

arranged on a glass metal plate to form a mold of desired size

A

leuckhart’s embedding mold

37
Q

mold for embedding:

consist of a series of interlocking plates resting on a flat metal base, forming several compartments

A

compound embedding unit

38
Q

mold for embedding:

has the advantage of embedding more specimens at a time

A

compound embedding unit

39
Q

mold for embedding:

used in positioning histological tissues accurately in base molds

A

plastic embedding ring and base mold

40
Q

mold for embedding:

disposable embedding mold

A

peel-a-way

41
Q

mold for embedding:

can be placed directly in the chuck of the microtome

A

peel-a-way

42
Q

mold for embedding:

made from thick paper or cardboard paper

A

paper boat

43
Q

mold for embedding:

cheap to make and allow blocks to be stored without being removed

A

paper boat

44
Q

mold for embedding:

provide easy and accurate identification of specimens, thereby avoiding confusion and interchange of tissue blocks

A

paper boat

45
Q

mold for embedding:

convenient molds for busy routine laboratory, one block being embedded in each compartment

A

plastic ice tray

46
Q

in using plastic ice tray, blocks are easily removed by flexing the plastic trays and by smearing the inside of the mold with ___ or ___ ___

A

glycerin
liquid paraffin

47
Q

mold for embedding:

ideal for embedding fragmentary biopsies

A

watch glass

48
Q

T/F: in using watch glass, it is essential to smear with glycerin as the blocks would be hard to remove

A

F (no need to smear)

49
Q

mold for embedding:

used for small fragments which have been processed (bone marrow) which concentrates them without the damage caused by orientation with forceps

A

test tube

50
Q

mold for embedding:

used for embedding tissue intended for EM microscopy

A

methacrylate plastic resin (EPON resin)

MT6321 (8 decks)

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