02 Dehydration Flashcards
pre-eminent type of tissue processing treatment considered to be the most suitable for routine preparation, sectioning, staining, and subsequent storage of large tissue samples
Paraffin wax method
paraffin wax method uses a series of ___ as dehydrating fluid
alcohol
the first step in tissue processing by which the intercellular and extracellular water from the tissue are removed after fixation and prior to wax infiltration
dehydration
solvents utilized in the removal of water
dehydrating agents/dehydrants
the removal of water from aqueaous-fixed tissue
dehydration
since most alcohols and paraffin are not miscible, another step, known as ___ is introduced
clearing
T/F: a dehydrating solution should evaporate very fast
F
It is important for a dehydrating solution to be able to dehydrate ___ tissues
fatty
T/F: a dehydrating solution should not harden tissues excessively and should remove stains
F (should NOT REMOVE stains)
dehydrating agent:
recommended for routine dehydration of tissues and considered the best dehydrating agent
ethyl alcohol
dehydrating agent:
clear, colorless, flammable fluid
ethyl alcohol
dehydrating agent:
primarily used for blood and tissue films, and for smear preparations
methyl alcohol
T/F: methanol is toxic to the body
T
T/F: ethanol is not poisonous
T
dehydrating agent:
utilized in plant and animal micro-techniques
butyl alcohol
dehydrating agent:
slow dehydrating agent producing less shrinkage that hardens less than ethanol
butyl alcohol
dehydrating agent:
not suitable for rapid tissue processing
butyl alcohol
in dehydrating, ___ (descending/ascending) concentrations of alcohol is used
ascending
factors affecting the strength of initial alcohol required in dehydration
size
nature of tissue
fixative used
smaller and more delicate tissues require ___ (higher/lower) concentration and ___ (longer/shorter) intervals between ascending grades of alcohol in dehydration
lower
shorter