02 Sectioning Flashcards
sectioning instrument that allows for the cutting of extremely thin slices of material called section
microtome
thickness of sections
4-15 um
unit for measurement for the thickness of sections
micron
a process where wax is removed with a sharp knife until about 2 mm remains on all sides of the tissues
block trimming
action of sharpening a knife by grinding cutting edge, either on a stone or with an abrasive compound
honing
a process of polishing the cutting edge of the knife on leather or canvas done after honing
stropping
the angle formed between the cutting edge of the microtome knife
bevel angle (27-32 degrees)
the angle formed between the surface of the block and the cutting facet of the knife
clearance angle (5-10 degrees)
the straight line formed by intersection of 2 planes, the cutting facets
cutting edge
radius of curvature of the cutting edge
0.1 to 0.35 um
length of cutting facets
0.1 to 0.6 mm
angle between the sides of the knives
wedge angle (15 degrees)
angle between upper surface of the cutting facet and the surface of the block
rake angle
the ___ (smaller/bigger) the bevel angle, the sharper the knife
smaller
ensures that only the cutting edge of the knife touches the specimen block
clearance angle
too much clearance causes the knife to ___
chatter
knife design and cut type:
extremely sharp, but are also very delicate and are therefore only used with very soft samples
planar concave
knife design and cut type:
more stable and find use in moderately hard materials, such as in epoxy or cryogenic sample cutting
wedge profile knives
knife design and cut type:
with its blunt edge, raises the stability of the knife, whilst requiring significantly more force to achieve the cut
chisel profile
knife design and cut type:
used on sliding, rotary, and rocking microtomes
plano-concave knife