Zoo lab 1 Flashcards
Label the microscope Top to Bottom
Ocular lenses,
Objective Lenses,
Stage with mechanical stage controls,
Condenser
Iris Diaphragm
Focusing Controls (coarse and fine)
Illumination
Stand (base and arm)
Procedure before using microscope?
Make sure the toggle is set to off before plugging in to power,
make sure illumination is at its lowest setting
Clean ocular and objective lenses
Working distance
Space between objective and coverglass
Parfocal
No focusing necessary when switching from one objective to another
Parcentric
No change in centering of the specimen is required between objective lens changes
Magnification
The times the object appears to be increased in length or diameter, when compared to its size from a fixed distance with the unaided eye (25 cm)
Contrast
Apparent difference in brightness between various parts of the specimen
Resolution
The measure of the amount of fine detail or sharpness that is apparent in the image of the specimen
Best resolving power is when…
The iris diaphragm is wide open
To increase contrast…
Add dye or slightly close iris diaphragm
Ocular lens is always… magnified
10x
Cheek cell
Nucleus
Cytoplasm
Plasma membrane
Which part of the cell stains most intensely with methylene blue?
The nucleus
Unfertilized sea star egg
Nucleolus
Chromatin granules (within the nucleus)
Large Nucleus
Nuclear membrane
Cytoplasm
Dissecting microscope labelled
(take picture in lab)
Stand
Ocular lens
Magnification knob
Stage
Focusing control
