X-Ray Crystallography Flashcards
1
Q
What are the advantages of X-ray crystallography?
A
- high structural resolution achieveable
- gold standard for drug screening/virtual screening
- co-crystals with ligand bound often possible
2
Q
What are the disadvantages of X-ray crystallography?
A
- Need a crystal
- Structure obtained is of protein in crystal state
- Unable to see H atoms
3
Q
What resolution is good to define atomic resolution?
A
2.5 Angstroms
4
Q
What resolution can still be useful to visualise molecules?
A
3-3.5 Angstroms
5
Q
Why is a crystal required to produce X-ray structures?
A
The regular structure enhances diffraction so makes rays more intense and easier to interpret
6
Q
What does the Fourier transform have?
A
Both magnitude and phase
