Cytoplasmic Dynein Mechanism Flashcards
Which direction does dynein move?
Towards minus end
What does dynein move along?
Microtubules
What is the function of dynein?
- Involved in transporting cellular cargo
- Providing forces and displacements important in mitosis
What is the general strutcture of dynein?
- Heavy chain heterdimer with multiple light chains
What binds cargo in dynein?
~160 kDa N-terminal domain which binds multiple light chains
In dynein what does dimerisation require?
Multiple diverse accessory subunits
How big is the motor domain of dynein?
~380 kDa
What is the structure of the motor in each heavy chain of dynein?
Contains 6AAA+ ATPase units which fold into a ring structure
What does the linker structure of dynein do during powerstroke?
Moves across the AAA+ ring
What extends from the AAA+ ring of dynein?
A coiled coil and microtubule binding site
What is the structure of an AAA+ module?
- Contains a large and small subunit domain
- Small subdomains pack against large domains of next module
how many ATPase sites are there on the AAA+ motor of dynein?
2
Which AAA modules of dynein possess walker A domain motifs?
AAA 1-4
What is the principal ATPase site in AAA+ motors of dynein?
AAA1
What is the function of AAA3 module of dynein motor?
Regulates communication between linker and AAA1