Transporters Flashcards
1
Q
Does ligand binding stabilise the dynamic flux of transporters?
A
No
2
Q
What discovery allowed lactose permease to be crystallised?
A
A lac permease mutant (C154G)
3
Q
Why was the C154G lactose permease mutant a useful discovery?
A
- It lacked the inward-facing conformation
- Had a higher thermal stability
- More robust
- Lacks extensive conformational dynamics of WT
- Binds sugar but does not transport it
4
Q
What was Lac permease crystallised in the presence of?
A
TDG
5
Q
Where is the ligand binding site in lac permease?
A
At the centre of the membrane
6
Q
How was the alternating access model of transporters generated?
A
The combination of experimental structures and computational modelling
7
Q
Which residues for hydrogen bonds with lactose permease?
A
- Arg144
- Lys358
