WHITE BLOOD CELLS DIFFERENTIAL

Question 1

What is the differential count of the following? include % and # of cells
1. PMN neutrophils
2. Bands
3. Eosinophil
4. Basophil
5. Lymphocytes
6. Monocytes

Answer 1

1. PMN 45- 75% 2000-8300
2. Bands 3-5% 130-570
3. Eosinophil 0-4% 0-450
4. Basophil 0-1 0-200
5. Lymphocytes 20-40 900-4,500
6. Monocytes 2-8 90-800

Question 2

what is the difference between left and rightshift

Answer 2

left shift is an increase in wbc immature cells (more bands and blasts) while right shift is the increase in number of mature cells in circulation

Question 3

what ids the clinical significance of left shift

Answer 3

it is observed in most infextions and in some cancers

Question 4

what is the clinical significance of right shift

Answer 4

it is seen in liver diseases, megaloblastic anaemias, haemolitic disorders and allerges

Question 5

explain the preparation of thich smear

Answer 5

1. obtain a drop of blood from EDTA bottle of from finger plick
2. place a drop of blood on the slide and spread it using a pasture pipete making a smear
3. the slide is left to dry

Question 6

explain how staining is done

Answer 6

stain the slide into fields stain A (methylene blue) it stains the nucleus to purple
wash the slide for 3 seconds, agitate gently

stain the slide into fields stain B (Eosin) that stains cytoplasm to pink/red for 3 seconds and wash gently withtap water

drai the slide and let it dry

Question 7

explain th examination of a thick smear

Answer 7

1. switch on the microscope and place the stained blood film on microscope satage
2. place a drop of immersion oil on the thick film
3. using x100 lens(oil immersion) focus on the blood firm
4. count up to 100 WBC and take note of white cell morphology
5. report the findings in percentage or absolute number