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HTL > What are they staining for? > Flashcards

What are they staining for? Flashcards

1
Q

Prussian Blue

A

Ferric Iron

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2
Q

Turnball Blue

A

Ferrous Iron (Fe2+)

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3
Q

Schmorl Technique

A

Reducing substances (melanin, argentaffin granules, and even formalin pigments)

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4
Q

Fontana Masson

A

Argentaffin substances (melanin, argentaffin granules of carcinoid tumors, and some neurosecretory granules)

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5
Q

Grimeluis

A

Argyrophil granules in neurosecretory tumors

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6
Q

Churukian-Schenk

A

Argyrophil granules in neurosecretory tumors

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7
Q

Gomori Methenamine Silver

A

Urates

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8
Q

Bile Stain

A

presence of bilirubin in tissue and to distinguish bile pigments from other pigments found in tissue

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9
Q

Von Kossa

A

presence of calcium in tissue

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10
Q

Alzarin Red S

A

calcium in tissue

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11
Q

Rhodanine

A

detection of copper in tissue, especially in liver, in Wilson disease

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12
Q

Kinyoun

A

acid-fast mycobacteria

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13
Q

Ziehl-Neelsen

A

acid-fast mycobacterium

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14
Q

Fite

A

detection of mycobacterium leprae (causative organism of leprosy)

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15
Q

Auramine-Rhodamine Fluorescence Technique

A

Mycobacterium tuberculosis and other acid-fast organisms

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16
Q

Brown-Hopps Modification

A

gram-negative and gram-positive bacteria

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17
Q

Modified Diff-Quik Giemsa

A

identification of H pylori

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18
Q

Alcian Yellow-Toluidine Blue

A

detection of H pylori

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19
Q

Hotchkiss-McManus PAS Reaction

A

demonstration of fungi

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20
Q

Chromic Acid-Schiff (CAS)

A

identification of fungi

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21
Q

Gridley

A

demonstration of fungi

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22
Q

Grocott Methenamine-Silver Nitrate

A

demonstration of fungal organisms

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23
Q

Microwave Methenamine-Silver Nitrate

A

rapid methoed most useful on cytospin prep or on frozen sections for the diagnosis of P jirovecii

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24
Q

Mayer Mucicarmine and Alcian Blue

A

can be used for mucoid capsule of C neoformans

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25
Q

Warthin Starry

A

demonstration of spirochetes

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26
Q

Dieterle Method

A

demonstration of spirochetes or the causative organism of legionellosis

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27
Q

Microwave Steiner and Steiner

A

demonstration of spirochetes, H pylori, or the causative organism of legionellosis

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28
Q

Cresyl Echt Violet Method I

A

identification of neurons in tissue sections, or demonstration of the loss of Nissl substance (chromatolysis)

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29
Q

Cresyl Echt Violet Method II

A

identification of neurons in tissue sections or the demonstration of loss of Nissl substance

30
Q

Bodian Method

A

nerve fibers

31
Q

Holmes Silver Nitrate

A

nerve fibers and neurofibrils

32
Q

Bielschowsky-PAS

A

nerve fibers and the presence of neurofibrillary tangles and senile plaques in Alzheimer disease

33
Q

Microwave Modification of Bielschowsky Method

A

nerve fibers and the presence of neurofibrillary tangles and senile plaques in Alzheimer disease

34
Q

Sevier-Munger Modification of Bielschowsky

A

nerve fibers and neurofibrillary tangles and senile plaques in Alzheimer disease

35
Q

Thioflavin S

A

neurofibrillary degeneration (neurofibrillary tangles, senile plaques, neurophil threads)

36
Q

Mallory Phosphotungstic Acid Hematoxylin (PTAH)

A

glial fibers

37
Q

Holzer Method

A

glial fibers and areas of gliosis

38
Q

Cajal Stain

A

astrocytes

39
Q

Weil Method

40
Q

Luxol Fast Blue

41
Q

Luxol Fast Blue-Cresyl Echt Violet

A

both myelin and Nissl substance

42
Q

Luxol Fast Blue-Holmes Silver Nitrate

A

myelin and nerve fibrers

43
Q

Luxol Fast Blue-PAS-Hematoxylin

A

myelin sheath, basement membranes, senile plaques, fungi, and corpora amylacea

44
Q

Masson Trichrome

A

differentiate between collagen and smooth muscle (in tumors and identify increases in collagenous tissue in diseases such as cirrhosis of the liver)

45
Q

Gomori 1-Step Trichrome

A

identification of increase in collagenous connective tissue fiber or the differentiation between collagen and smooth muscle

46
Q

Van Gieson Picric Acid-Acid Fuschin

A

connective tissue

47
Q

Verhoeff

A

pathologic changes in elastic fibers

48
Q

Aldehyde Fuchsin

A

pathologic changes to elastic fibers

49
Q

Russel Modification of the Movat Pentachrome

A

mucin, fibrin, elastic fibers, muscle and collagen

50
Q

Gomori

A

reticular fibers

51
Q

Gordon and Sweets

A

reticular fibers

52
Q

Mallory PTAH

A

muscle cross-striation and fibrin

53
Q

Periodic Acid-Methenamine Silver

A

delineates basement membranes

54
Q

Oil Red O

A

neutral lipids (FAT)

55
Q

Sudan Black B in Propylene Glycol

A

neutral lipids

56
Q

Osmium Tetroxide

A

demonstration of fat that allows paraffin embedded tissues

57
Q

Toluidine Blue

A

Mast cells

58
Q

PAS

A

polysaccharides, neutral mucosubstances, and basement membranes

59
Q

PAS with Diastase Digestion

60
Q

Best Carmine

61
Q

Mayer Mucicarmine

A

“epithelial” mucin

62
Q

Alcian Blue (pH 2.5)

A

acid mucopolysaccharides

63
Q

Alcian Blue (pH 1)

A

sulfated mucosubstances

64
Q

Alcian Blue with Hyaluronidase

A

differentiation between epithelial and connective tissue mucins

65
Q

Alcian Blue-PAS-Hematoxylin

A

differentiation between neutral and acidic mucosubstances

66
Q

Muller-Mowry Colloidal Iron

A

carboxylated and sulfated mucopolysaccharides and glycoproteins

67
Q

Alkaline Congo Red

68
Q

Crystal Violet

69
Q

Thioflavin T Fluorescent

70
Q

Feulgen

71
Q

Methyl-Green-Pyronin Y

A

differentiate between DNA and RNA

72
Q

May-Grunwald Giemsa

A

differentiation of cells present in hematopoietic tissue

HTL (18 decks)

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