What are they staining for? Flashcards
Prussian Blue
Ferric Iron
Turnball Blue
Ferrous Iron (Fe2+)
Schmorl Technique
Reducing substances (melanin, argentaffin granules, and even formalin pigments)
Fontana Masson
Argentaffin substances (melanin, argentaffin granules of carcinoid tumors, and some neurosecretory granules)
Grimeluis
Argyrophil granules in neurosecretory tumors
Churukian-Schenk
Argyrophil granules in neurosecretory tumors
Gomori Methenamine Silver
Urates
Bile Stain
presence of bilirubin in tissue and to distinguish bile pigments from other pigments found in tissue
Von Kossa
presence of calcium in tissue
Alzarin Red S
calcium in tissue
Rhodanine
detection of copper in tissue, especially in liver, in Wilson disease
Kinyoun
acid-fast mycobacteria
Ziehl-Neelsen
acid-fast mycobacterium
Fite
detection of mycobacterium leprae (causative organism of leprosy)
Auramine-Rhodamine Fluorescence Technique
Mycobacterium tuberculosis and other acid-fast organisms
Brown-Hopps Modification
gram-negative and gram-positive bacteria
Modified Diff-Quik Giemsa
identification of H pylori
Alcian Yellow-Toluidine Blue
detection of H pylori
Hotchkiss-McManus PAS Reaction
demonstration of fungi
Chromic Acid-Schiff (CAS)
identification of fungi
Gridley
demonstration of fungi
Grocott Methenamine-Silver Nitrate
demonstration of fungal organisms
Microwave Methenamine-Silver Nitrate
rapid methoed most useful on cytospin prep or on frozen sections for the diagnosis of P jirovecii
Mayer Mucicarmine and Alcian Blue
can be used for mucoid capsule of C neoformans
Warthin Starry
demonstration of spirochetes
Dieterle Method
demonstration of spirochetes or the causative organism of legionellosis
Microwave Steiner and Steiner
demonstration of spirochetes, H pylori, or the causative organism of legionellosis
Cresyl Echt Violet Method I
identification of neurons in tissue sections, or demonstration of the loss of Nissl substance (chromatolysis)