Enzyme Histochemistry

Question 1

Enzymes are:

Answer 1

proteins

Question 2

Biological oxidation occurs with the:

Answer 2

loss of hydrogen or gain or oxygen

Question 3

A compound is reduced when what is removed?

Answer 3

oxygen

Question 4

The compound or chemical group on which a specific enzyme works is known as its:

Answer 4

substrate

Question 5

Tissue for some enzyme demonstration should be fixed if possible because:

Answer 5

diffusion artifact is decreased

Question 6

Tissue for enzyme studies has been fixed in cold calcium formalin. The best storage medium is:

Answer 6

30% sucrose with 1% gum acacia

Question 7

Enzymes that act on substrates by adding water belong to a group known as:

Answer 7

hydrolases

Question 8

The optimum procedure for freezing muscle biopsies is:

Answer 8

isopentane at -150

Question 9

The supply of isopentane is depleted, although liquid nitrogen is available. The best method for freezing a muscle biopsy is:

Answer 9

dust with talc, the freeze with liquid nitrogen

Question 10

The most frequently used method of demonstrating hydrolytic enzymes involve:

Answer 10

simultaneous coupling