Carbohydrates Flashcards
Glycogen is best demonstrated by the use of:
a. crystal violet colloidal iron
b. Mayer mucicarmine
c. PAS with and without diastase
d. alcian blue with and without hyaluronidase
c. PAS with and without diastase
Amyloid can be demonstrated with:
a. Congo red
b. Mayer mucicarmine
c. cresyl echt violet
d. alcian blue
a. congo red
The Schiff reaction demonstrates
aldehydes
A good control for mayer mucicarmine stain is:
appendix (as it stains goblet cells)
A good control for glycogen is:
liver
To increase the specificity for amyloid, Congo red stains should be examined by what type of microscopy?
Polarized
Of the following, which is the best fixative for glycogen?
a. Zenker
b. Orth
c. Acetic acid
d. Absolute alcohol
d. absolute alcohol
Periodic acid us used in the PAS technique as a/an:
oxidizer
The crystal violet stain for amyloid is a/an:
polychromatic stain
Acid mucopolysaccharides are demonstrated by:
alcian blue
The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of the Schiff solution?
Formaldehyde
A fluorescent dye used to demonstrate amyloid is:
Thioflavin T
The alcian blue stain performed at 1.0 pH dmeonstrates:
sulfated mucosubstances
Substances stained positive with the colloidal iron procedure will be what color?
blue
Schiff reagent is a/an:
a. reduced solution of basic fuchsin
b. oxidized solution of basic fuchsin
c. reduced solution of acid fuchsin
d. oxidized solution of acid fuchsin
a. reduced solution of basic fuchsin
Diastase digestion increases specificity for:
glycogen
Colloidal iron is used for the demonstration of:
acid mucopolysaccharides
Which of the following methods will demonstrate glycogen?
a. Congo red
b. Giemsa
c. Mayer Mucicarmine
d. Best carmine
d. best carmine
Adjacent sections are stained with PAS, one with and one without diastase digestion. A positive result on the one without digestion and a negative result on the one with digestion indicate the presence of:
glycogen
What type of fungi is demonstrated with the colloidal iron stain?
Cryptococcus neoformans
No staining of the glomerular membrane can be seen microscopically on a control section of kidney. This could be due to:
inadequate oxidation
Marked nonspecific background staining is noted on a section stained with PAS technique. This could be the result of:
fixation in gluteraldehyde
Very weak staining is noted on a PAS-stained control section of liver. One problem solving action is to:
check the schiff reagent with formaldehyde
Section of small intestine show orange goblet cells that are partially obscured by the yellow background. This is most likely the result of:
overstaining with metanil yellow
Control sections stained with Congo Red show only yellow, no green, birefringence. This could probably be corrected in the future by:
ensuring sections are cut at 8-10 micrometers
