Carbohydrates

Question 1

Glycogen is best demonstrated by the use of:

a. crystal violet colloidal iron
b. Mayer mucicarmine
c. PAS with and without diastase
d. alcian blue with and without hyaluronidase

Answer 1

c. PAS with and without diastase

Question 2

Amyloid can be demonstrated with:

a. Congo red
b. Mayer mucicarmine
c. cresyl echt violet
d. alcian blue

Answer 2

a. congo red

Question 3

The Schiff reaction demonstrates

Answer 3

aldehydes

Question 4

A good control for mayer mucicarmine stain is:

Answer 4

appendix (as it stains goblet cells)

Question 5

A good control for glycogen is:

Answer 5

liver

Question 6

To increase the specificity for amyloid, Congo red stains should be examined by what type of microscopy?

Answer 6

Polarized

Question 7

Of the following, which is the best fixative for glycogen?

a. Zenker
b. Orth
c. Acetic acid
d. Absolute alcohol

Answer 7

d. absolute alcohol

Question 8

Periodic acid us used in the PAS technique as a/an:

Answer 8

oxidizer

Question 9

The crystal violet stain for amyloid is a/an:

Answer 9

polychromatic stain

Question 10

Acid mucopolysaccharides are demonstrated by:

Answer 10

alcian blue

Question 11

The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of the Schiff solution?

Answer 11

Formaldehyde

Question 12

A fluorescent dye used to demonstrate amyloid is:

Answer 12

Thioflavin T

Question 13

The alcian blue stain performed at 1.0 pH dmeonstrates:

Answer 13

sulfated mucosubstances

Question 14

Substances stained positive with the colloidal iron procedure will be what color?

Answer 14

blue

Question 15

Schiff reagent is a/an:

a. reduced solution of basic fuchsin
b. oxidized solution of basic fuchsin
c. reduced solution of acid fuchsin
d. oxidized solution of acid fuchsin

Answer 15

a. reduced solution of basic fuchsin

Question 16

Diastase digestion increases specificity for:

Answer 16

glycogen

Question 17

Colloidal iron is used for the demonstration of:

Answer 17

acid mucopolysaccharides

Question 18

Which of the following methods will demonstrate glycogen?

a. Congo red
b. Giemsa
c. Mayer Mucicarmine
d. Best carmine

Answer 18

d. best carmine

Question 19

Adjacent sections are stained with PAS, one with and one without diastase digestion. A positive result on the one without digestion and a negative result on the one with digestion indicate the presence of:

Answer 19

glycogen

Question 20

What type of fungi is demonstrated with the colloidal iron stain?

Answer 20

Cryptococcus neoformans

Question 21

No staining of the glomerular membrane can be seen microscopically on a control section of kidney. This could be due to:

Answer 21

inadequate oxidation

Question 22

Marked nonspecific background staining is noted on a section stained with PAS technique. This could be the result of:

Answer 22

fixation in gluteraldehyde

Question 23

Very weak staining is noted on a PAS-stained control section of liver. One problem solving action is to:

Answer 23

check the schiff reagent with formaldehyde

Question 24

Section of small intestine show orange goblet cells that are partially obscured by the yellow background. This is most likely the result of:

Answer 24

overstaining with metanil yellow

Question 25

Control sections stained with Congo Red show only yellow, no green, birefringence. This could probably be corrected in the future by:

Answer 25

ensuring sections are cut at 8-10 micrometers